Submit a Review & Earn an Amazon Gift Card
You can now submit reviews for your favorite Tocris products. Your review will help other researchers decide on the best products for their research. Why not submit a review today?!
Submit ReviewWS 6
Description: Induces growth and proliferation of pancreatic islet cells, murine totipotent stem cells, GABAergic neurons and hippocampal neurons
Chemical Name: 4-[[6-[(Cyclopropylcarbonyl)amino]-4-pyrimidinyl]oxy]-N-[4-[(4-methyl-1-piperazinyl)methyl]-3-(trifluoromethyl)phenyl]benzeneacetamide
Purity: ≥98% (HPLC)
Biological Activity for WS 6
WS 6 induces growth and proliferation of cultured islet α and β cells in vitro (EC50 = 0.28 μM at β cells). Inhibits growth of FLT3-ITD+ AML and K562 CML cells (IC50 values are <0.24 nM and 40.48 nM, respectively). Reduces blood glucose in an animal model of type 1 diabetes. In combination with TTNPB (Cat. No. 0761) and 1-azakenpaullone, WS 6 induces and maintains murine totipotent stem cells. In an animal model of depression WS 6 induces dendrite complexity, enhances hippocampal neurogenesis, increases GABAergic neurons and GABA expression, and displays antidepressant and anxiolytic activity. WS 6 is predicted to be a receptor tyrosine kinase inhibitor.
Technical Data for WS 6
| M. Wt | 568.59 |
| Formula | C29H31F3N6O3 |
| Storage | Store at -20°C |
| Purity | ≥98% (HPLC) |
| CAS Number | 1421227-53-3 |
| PubChem ID | 71566751 |
| InChI Key | FTODTDQFHDJWIQ-UHFFFAOYSA-N |
| Smiles | O=C(NC1=CC=C(C(=C1)C(F)(F)F)CN2CCN(C)CC2)CC3=CC=C(OC4=NC=NC(=C4)NC(=O)C5CC5)C=C3 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for WS 6
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 56.86 | 100 | |
| ethanol | 56.86 | 100 |
Preparing Stock Solutions for WS 6
The following data is based on the product molecular weight 568.59. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.76 mL | 8.79 mL | 17.59 mL |
| 5 mM | 0.35 mL | 1.76 mL | 3.52 mL |
| 10 mM | 0.18 mL | 0.88 mL | 1.76 mL |
| 50 mM | 0.04 mL | 0.18 mL | 0.35 mL |
Molarity Calculator
Molarity Calculator
Calculate the mass, volume, or concentration required for a solution.
Reconstitution Calculator
Reconstitution Calculator
Dilution Calculator
Dilution Calculator
Calculate the dilution required to prepare a stock solution.
Product Datasheets for WS 6
References for WS 6
References are publications that support the biological activity of the product.
Hu et al (2022) Induction of mouse totipotent stem cells by a defined chemical cocktail. Nature PMID: 35728625
Zhang et al (2021) WS6 induces adult hippocampal neurogenesis in correlation to its antidepressant effect on the alleviation of depressive-like behaviors of rats. Neuroscience 473 119 PMID: 34455011
Menhaji-Klotz and Price (2015) Small molecules that promote regenerative repair for pancreatic and cardiovascular health. Bioorg.Med.Chem.Lett. 25 5465 PMID: 26508547
Shen et al (2013) Small-molecule inducer of β cell proliferation identified by high-throughput screening. J.Am.Chem.Soc. 135 1669 PMID: 23330637
Bregante et al (2021) Efficacy and synergy of small molecule inhibitors targeting FLT3-ITD+ acute myeloid leukemia. Cancers (Basel) 13 6181 PMID: 34944800
If you know of a relevant reference for WS 6, please let us know.
View Related Products by Target
Keywords: WS 6, WS 6 supplier, WS6, islet, beta, alpha, cells, proliferation, stem, AML, CML, myeloid, leukemia, diabetes, antidepressant, depression, anxiety, hippocampal, neurogenesis, GABA, growth, inhibitor, inhibitors, inhibit, inhibits, Stem, Cell, Proliferation, Other, RTKs, 7791, Tocris Bioscience
Citations for WS 6
Citations are publications that use Tocris products.
Currently there are no citations for WS 6. Do you know of a great paper that uses WS 6 from Tocris? Please let us know.
Reviews for WS 6
There are currently no reviews for this product. Be the first to review WS 6 and earn rewards!
Have you used WS 6?
Submit a review and receive an Amazon gift card.
$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Literature in this Area
Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!
*Please note that Tocris will only send literature to established scientific business / institute addresses.
Stem Cell Research Product Guide
This product guide provides a background to the use of small molecules in stem cell research and lists over 200 products for use in:
- Self-renewal and Maintenance
- Differentiation
- Reprogramming
- Organoid Generation
- GMP and Ancillary Material Grade Products
Stem Cells Scientific Review
Written by Kirsty E. Clarke, Victoria B. Christie, Andy Whiting and Stefan A. Przyborski, this review provides an overview of the use of small molecules in the control of stem cell growth and differentiation. Key signaling pathways are highlighted, and the regulation of ES cell self-renewal and somatic cell reprogramming is discussed. Compounds available from Tocris are listed.
Stem Cell Workflow Poster
Stem cells have potential as a source of cells and tissues for research and treatment of disease. This poster summarizes some key protocols demonstrating the use of small molecules across the stem cell workflow, from reprogramming, through self-renewal, storage and differentiation to verification. Advantages of using small molecules are also highlighted.
Stem Cells Poster
Written by Rebecca Quelch and Stefan Przyborski from Durham University (UK), this poster describes the isolation of pluripotent stem cells, their maintenance in culture, differentiation, and the generation and potential uses of organoids.