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Submit Reviewα-Amanitin
说明： Inhibitor of RNA polymerase II
化学名： cyclo[L-Asparaginyl-4-hydroxy-L-proly-(R-4,5-dihydroxy-L-isoleucyl-6-hydroxy-2-mercapto-L-tryptophylglycyl-L-isoleucylglycyl-L-cysteinyl]cyclic (4→8)-sulfide (R)-S-oxide
纯度： ≥95% (HPLC)
生物活性 for α-Amanitin
α-Amanitin is an inhibitor of RNA polymerase II. Inhibits transcription in eukaryotic cells. Binds and blocks the largest subunits of RNA polymerase II, preventing new ribonucleotides from incorporating into the nascent RNA chain. Potent amatoxin.
技术数据 for α-Amanitin
| 分子量 | 918.97 |
| 公式 | C39H54N10O14S |
| 储存 | Store at -20°C |
| 纯度 | ≥95% (HPLC) |
| CAS Number | 23109-05-9 |
| PubChem ID | 441541 |
| InChI Key | CIORWBWIBBPXCG-JAXJKTSHSA-N |
| Smiles | O=[C@@](N[C@@](C(N[C@@H](CC2=C(S4=O)NC3=C2C=CC(O)=C3)C(NCC(N[C@]([H])5[C@@H](C)CC)=O)=O)=O)([H])[C@@H](C)[C@@H](O)CO)[C@@H]1C[C@@H](O)CN1C([C@H](CC(N)=O)NC([C@H](C4)NC(CNC5=O)=O)=O)=O |
上方提供的技术数据仅供参考。批次相关数据请参见分析证书。
Tocris products are intended for laboratory research use only, unless stated otherwise.
溶解性数据 for α-Amanitin
| 溶剂 | 最高浓度 mg/mL | 最高浓度 mM | |
|---|---|---|---|
| 溶解性 | |||
| water | 4.59 | 5 |
制备储备液 for α-Amanitin
以下数据基于产品分子量 918.97。 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| 浓度/溶剂体积/质量 | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.05 mM | 21.76 mL | 108.82 mL | 217.63 mL |
| 0.25 mM | 4.35 mL | 21.76 mL | 43.53 mL |
| 0.5 mM | 2.18 mL | 10.88 mL | 21.76 mL |
| 2.5 mM | 0.44 mL | 2.18 mL | 4.35 mL |
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产品说明书 for α-Amanitin
参考文献 for α-Amanitin
参考文献是支持产品生物活性的出版物。
Casse et al (1999) The transcriptional inhibitors, actinomycin D and α-amanitin, activate the HIV-1 promoter and favor phosphorylation of the RNA polymerase II C-terminal domain. J.Biol.Chem. 274 16097 PMID: 10347161
Raha et al (2010) Close association of RNA polymerase II and many transcription factors with Pol III genes. Proc.Natl.Acad.Sci. 107 3639
If you know of a relevant reference for α-Amanitin, please let us know.
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关键词: alpha-Amanitin, alpha-Amanitin supplier, alpha-Amanitin, a-Amanitin, α-Amanitin, RNA, polymerases, II, inhibitors, inhibits, DNA,, and, Protein, Synthesis, Polymerase, 4025, Tocris Bioscience
4 篇 α-Amanitin 的引用文献
引用文献是使用了 Tocris 产品的出版物。 α-Amanitin 的部分引用包括：
Feng et al (2021) Loss of centromeric RNA activates the spindle assembly checkpoint in mammalian female meiosis I. J Cell Biol 220 PMID: 34379093
Jing et al (2019) Remodeling of Interstrand Crosslink Proximal Replisomes Is Dependent on ATR, FANCM, and FANCD2. Cell Rep 27 1794-1808.e5 PMID: 31067464
Rafael et al (2022) BRD2 compartmentalizes the accessible genome. Nat Genet 54 481-491 PMID: 35410381
Philip R et al (2020) HIV-1 replication complexes accumulate in nuclear speckles and integrate into speckle-associated genomic domains. Nat Commun 11 3505 PMID: 32665593
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α-Amanitin 的评论
平均评分: 5 (Based on 1 Review.)
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alpha -Amanitin was used in antibacterial resistance experiments.
By
Anonymous on 02/20/2019
种属： Human
alpha -Amanitin was used in antibacterial resistance experiments.We looked into RNAP translocation along the DNA without dissociating from either the template or the growing RNA product until it encounters a termination factor or signal that causes the transcription cycle end with dissociation of the transcribing complex and release of RNA polymerase for a new round of transcription.
