Brefeldin A

Name: Brefeldin A
Brand: Tocris Bioscience
SKU: 1231
Rating: 5 (1 reviews)

Cat. No. 1231 10 篇引用文献 1 条评论提交评论说明书 / 分析证书（CoA） / 化学品安全技术说明书（SDS）

Pricing	Availability		Qty

说明： Disrupts protein translocation to Golgi

化学名： 1,6,7,8,9,11aβ,12,13,14,14aα-Decahydro-1β,13α-dihydroxy-6β-methyl-4H-cyclopent[f]oxacyclotridecin-4-one

纯度： ≥98% (HPLC)

说明书

引用文献 (10)

生物活性技术数据溶解性计算器说明书参考文献

生物活性 for Brefeldin A

Brefeldin A is a reversible inhibitor of protein translocation from the endoplasmic reticulum (ER) to the Golgi apparatus. Blocks binding of ADP-ribosylation factor (ARF1) to the Golgi apparatus and inhibits GDP-GTP exchange, leading to activation of ER stress signaling pathways. Can be used to induce autophagy in mammalian cells. Also enhances CRISPR-mediated homology-directed repair (HDR) efficiency ~2-fold when applied at 100 nM, in human induced pluripotent stem cells (iPSCs). Antifungal.

技术数据 for Brefeldin A

分子量	280.36
公式	C₁₆H₂₄O₄
储存	Store at -20°C
纯度	≥98% (HPLC)
CAS Number	20350-15-6
PubChem ID	6436187
InChI Key	KQNZDYYTLMIZCT-SZXCEISTSA-N
Smiles	O[C@@H]([C@H]1[C@H](/C=C/CCC[C@H](C)O2)C[C@H](O)C1)/C=C/C2=O

上方提供的技术数据仅供参考。批次相关数据请参见分析证书。

Tocris products are intended for laboratory research use only, unless stated otherwise.

溶解性数据 for Brefeldin A

	溶剂	最高浓度 mg/mL	最高浓度 mM
溶解性
溶解性	DMSO	14.02	50

制备储备液 for Brefeldin A

以下数据基于产品分子量 280.36。 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

浓度/溶剂体积/质量	1 mg	5 mg	10 mg
0.5 mM	7.13 mL	35.67 mL	71.34 mL
2.5 mM	1.43 mL	7.13 mL	14.27 mL
5 mM	0.71 mL	3.57 mL	7.13 mL
25 mM	0.14 mL	0.71 mL	1.43 mL

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产品说明书 for Brefeldin A

分析证书/产品说明书

化学品安全技术说明书

参考文献 for Brefeldin A

参考文献是支持产品生物活性的出版物。

Ktistakis et al (1992) Action of brefeldin A blocked by activation of a pertussis-toxin-sensitive G protein. Nature 356 344 PMID: 1549178

Morinaga et al (1996) Isolation of a brefeldin A-inhibited guanine nucleotide-exchange protein for ADP ribosylation factor (ARF)1 and ARF3 that contains a Sec7-like domain. Proc.Natl.Acad.Sci.U.S.A. 93 12856 PMID: 8917509

Tsai et al (1994) Identification of a brefeldin A-insensitive guanine nucleotide-exchange protein for ADP-ribosylation factor in bovine brain. Proc.Natl.Acad.Sci.U.S.A. 91 3063 PMID: 8159707

Ding et al (2007) Differential effects of endoplasmic reticulum stress-induced autophagy on cell survival. J.Biol.Chem. 282 4702 PMID: 17135238

Yu et al (2015) Small molecules enhance CRISPR genome editing in pluripotent stem cells. Cell Stem Cell 16 142 PMID: 25658371

Oh-Hashi et al (2021) Elucidation of brefeldin A-induced ER and Golgi stress responses in Neuro2a cells. Mol. Cell. Biochem. 476 3869 PMID: 34129155

If you know of a relevant reference for Brefeldin A, please let us know.

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