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Submit ReviewJanelia Fluor® 525, Haloalkane 最新
说明： Cell-permeable fluorogenic fluorescent dye with a chloroalkane handle. Application: live-cell imaging as a self-labeling tag substrate
化学名： 4-((2-(2-((6-Chlorohexyl)oxy)ethoxy)ethyl)carbamoyl)-2-(3-(3,3-difluoroazetidin-1-ium-1-ylidene)-6-(3,3-difluoroazetidin-1-yl)-3H-xanthen-9-yl)benzoate
纯度： ≥95% (HPLC)
生物活性 for Janelia Fluor® 525, Haloalkane
Key Information: Janelia Fluor® 525, Haloalkane is a yellow cell-permeable fluorogenic fluorescent dye with a chloroalkane handle.
Application: Live-cell imaging as a self-labeling tag substrate. Suitable for confocal microscopy, light sheet microscropy, super resolution microscopy (SRM) techniques including dSTORM (in both live and fixed cells). Stable to fixation. Cell permeable.
Properties and Photophysical Data: Janelia Fluor® 525, Haloalkane shows a high degree of fluorogenicity. Excitation and emission maxima (λ) are 525 nm and 549 nm, respectively; quantum yield = 0.91; extinction coefficient = 122,000 M-1cm-1 (measured in ethanol or TFE plus 0.1% TFA); lactone-zwitterion equilibrium constant (KL-Z) = 0.068. Exhibits low non-specific background staining.
Janelia Fluor is a registered trademark of Howard Hughes Medical Institute.
许可信息
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus
Optical Data for Janelia Fluor® 525, Haloalkane
| Emission Color | Yellow |
|---|---|
| λabs | 525 nm |
| λem | 549 nm |
| Extinction Coefficient (ε) | 122,000 M-1cm-1 |
| Quantum Yield (φ) | 0.91 |
| Closest Laser line | 488 nm |
| Cell Permeable | Yes |
| Application | Confocal Microscopy, Super Resolution Microscopy (SRM) |
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技术数据 for Janelia Fluor® 525, Haloalkane
| 分子量 | 732.17 |
| 公式 | C37H38ClF4N3O6 |
| 储存 | Store at -20°C |
| 纯度 | ≥95% (HPLC) |
| CAS Number | 1811539-37-3 |
| PubChem ID | 140772613 |
| InChI Key | TZCOVYGWYYWODF-UHFFFAOYSA-N |
| Smiles | O=C(C1=C(C(C2=C(OC3=C/4)C=C(N5CC(F)(C5)F)C=C2)=C3C=CC4=[N+]6CC(F)(C\6)F)C=C(C(NCCOCCOCCCCCCCl)=O)C=C1)[O-] |
上方提供的技术数据仅供参考。批次相关数据请参见分析证书。
Tocris products are intended for laboratory research use only, unless stated otherwise.
溶解性数据 for Janelia Fluor® 525, Haloalkane
| 溶剂 | 最高浓度 mg/mL | 最高浓度 mM | |
|---|---|---|---|
| 溶解性 | |||
| DMSO | 7.32 | 10 |
制备储备液 for Janelia Fluor® 525, Haloalkane
以下数据基于产品分子量 732.17。 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| 浓度/溶剂体积/质量 | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.1 mM | 13.66 mL | 68.29 mL | 136.58 mL |
| 0.5 mM | 2.73 mL | 13.66 mL | 27.32 mL |
| 1 mM | 1.37 mL | 6.83 mL | 13.66 mL |
| 5 mM | 0.27 mL | 1.37 mL | 2.73 mL |
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产品说明书 for Janelia Fluor® 525, Haloalkane
参考文献 for Janelia Fluor® 525, Haloalkane
参考文献是支持产品生物活性的出版物。
Grimm et al (2017) A general method to fine-tune fluorophores for live-cell and in vivo imaging. Nat. Methods 17 987 PMID: 28869757
If you know of a relevant reference for Janelia Fluor® 525, Haloalkane, please let us know.
关键词: Janelia Fluor® 525, Haloalkane, Janelia Fluor® 525, Haloalkane supplier, Janelia, Fluor525-haloalkane, JF525, fluorescent, fluorogenic, confocal, light, sheet, cell, permeable, super, resolution, microscopy, dyes, live, imaging, SRM, dSTORM, Haloalkane, Fluor, Dyes, Self-labeling, Tags, 8805, Tocris Bioscience
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