Submit a Review & Earn an Amazon Gift Card
You can now submit reviews for your favorite Tocris products. Your review will help other researchers decide on the best products for their research. Why not submit a review today?!
Submit ReviewJanelia Fluor® 635, Haloalkane 最新
说明： Cell-permeable fluorogenic fluorescent dye with a chloroalkane handle. Application: live-cell imaging as a self-labeling tag substrate
化学名： 4-((2-(2-((6-Chlorohexyl)oxy)ethoxy)ethyl)carbamoyl)-2-(3-(3-fluoroazetidin-1-ium-1-ylidene)-7-(3-fluoroazetidin-1-yl)-5,5-dimethyl-3,5-dihydrodibenzo[b,e]silin-10-yl)benzoate
纯度： ≥95% (HPLC)
生物活性 for Janelia Fluor® 635, Haloalkane
Key Information: Janelia Fluor® 635, Haloalkane is a red cell-permeable fluorogenic fluorescent dye with a chloroalkane handle.
Application: Live-cell imaging as a self-labeling tag substrate. Suitable for confocal microscopy, light sheet microscropy, super resolution microscopy (SRM) techniques including dSTORM (in both live and fixed cells). Stable to fixation. Cell permeable.
Properties and Photophysical Data: Janelia Fluor® 635, Haloalkane shows a high degree of fluorogenicity. Excitation and emission maxima (λ) are 635 nm and 652 nm, respectively; quantum yield = 0.56; extinction coefficient = 167,000 M-1cm-1 (measured in ethanol or TFE plus 0.1% TFA); lactone-zwitterion equilibrium constant (KL-Z) <0.0001. Turn-on ratio in vitro A/A0 = 113, F/F0 = 67. Exhibits low non-specific background staining.
Janelia Fluor is a registered trademark of Howard Hughes Medical Institute.
许可信息
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus
Optical Data for Janelia Fluor® 635, Haloalkane
| Emission Color | Red |
|---|---|
| Brightness | |
| λabs | 635 nm |
| λem | 652 nm |
| Extinction Coefficient (ε) | 167000 M-1cm-1 |
| Quantum Yield (φ) | 0.56 |
| Closest Laser line | 635 nm |
| Cell Permeable | Yes |
| Application | Confocal Microscopy, Super Resolution Microscopy (SRM) |
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectral Viewer
技术数据 for Janelia Fluor® 635, Haloalkane
| 分子量 | 738.34 |
| 公式 | C39H46ClF2N3O5Si |
| 储存 | Store at -20°C |
| 纯度 | ≥95% (HPLC) |
| CAS Number | 1811539-66-8 |
| PubChem ID | 140879857 |
| InChI Key | SSLCQPFUOBJTQH-UHFFFAOYSA-N |
| Smiles | O=C(NCCOCCOCCCCCCCl)C1=CC=C(C([O-])=O)C(C(C2=CC=C(N3CC(F)C3)C=C2[Si]4(C)C)=C(C=C/5)C4=CC5=[N+]6CC(F)C\6)=C1 |
上方提供的技术数据仅供参考。批次相关数据请参见分析证书。
Tocris products are intended for laboratory research use only, unless stated otherwise.
溶解性数据 for Janelia Fluor® 635, Haloalkane
| 溶剂 | 最高浓度 mg/mL | 最高浓度 mM | |
|---|---|---|---|
| 溶解性 | |||
| DMSO | 7.38 | 10 |
制备储备液 for Janelia Fluor® 635, Haloalkane
以下数据基于产品分子量 738.34。 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| 浓度/溶剂体积/质量 | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.1 mM | 13.54 mL | 67.72 mL | 135.44 mL |
| 0.5 mM | 2.71 mL | 13.54 mL | 27.09 mL |
| 1 mM | 1.35 mL | 6.77 mL | 13.54 mL |
| 5 mM | 0.27 mL | 1.35 mL | 2.71 mL |
Molarity Calculator
Molarity Calculator
Calculate the mass, volume, or concentration required for a solution.
Reconstitution Calculator
Reconstitution Calculator
Dilution Calculator
Dilution Calculator
Calculate the dilution required to prepare a stock solution.
产品说明书 for Janelia Fluor® 635, Haloalkane
参考文献 for Janelia Fluor® 635, Haloalkane
参考文献是支持产品生物活性的出版物。
Grimm et al (2017) A general method to fine-tune fluorophores for live-cell and in vivo imaging. Nat. Methods 17 987 PMID: 28869757
Cook et al (2023) HaloTag-based reporters for fluorescence imaging and biosensing. Chembiochem. 24 e202300022 PMID: 36815462
Tsai et al (2017) Nuclear microenvironments modulate transcription from low-affinity enhancers. Elife 6 e28975 PMID: 29095143
If you know of a relevant reference for Janelia Fluor® 635, Haloalkane, please let us know.
关键词: Janelia Fluor® 635, Haloalkane, Janelia Fluor® 635, Haloalkane supplier, Janelia, Fluor635-haloalkane, JF635, fluorescent, fluorogenic, confocal, light, sheet, cell, permeable, super, resolution, microscopy, red, dyes, live, imaging, SRM, dSTORM, HaloTag, Haloalkane, Fluor, Dyes, Self-labeling, Tags, 8808, Tocris Bioscience
篇 Janelia Fluor® 635, Haloalkane 的引用文献
引用文献是使用了 Tocris 产品的出版物。
目前没有 Janelia Fluor® 635, Haloalkane 的引用文献。 您是否知道使用了 Tocris Janelia Fluor® 635, Haloalkane 的优秀论文？ 请告知我们.
Janelia Fluor® 635, Haloalkane 的评论
目前没有该产品的评论。 Be the first to review Janelia Fluor® 635, Haloalkane and earn rewards!
Have you used Janelia Fluor® 635, Haloalkane?
Submit a review and receive an Amazon gift card.
$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image