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Submit ReviewTFAX 546, SE is an amine reactive yellow fluorescent dye. Insensitive to pH in the range 4 - 10. Forms bright and photostable conjugates with proteins and antibodies. Suitable for use in microscopy. Excitation maximum = 554 nm; emission maximum = 570 nm; extinction coefficient = 112,000 M-1cm-1; quantum yield = 0.79.
Emission Color | Yellow |
---|---|
Brightness | |
λabs | 554 nm |
λem | 570 nm |
Extinction Coefficient (ε) | 112000 M-1cm-1 |
Quantum Yield (φ) | 0.79 |
Closest Laser line | 532 nm |
Reactive Group | NHS ester |
Reactivity | Primary amines |
Cell Permeable | No |
pH Sensitivity | pH insensitive pH 4-10 |
Application | microscopy |
Alternative For | TMR, Cyanine3, Alexa Fluor 546 SE, AF 546 SE |
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectral Viewer分子量 | 1159.6 |
公式 | C44H47Cl3N4O14S.(CH3CH2)3NH |
储存 | Store at -20°C |
Smiles | ClC(C(Cl)=C1C(O)=O)=C(SCC(NCCCCCC(ON2C(CCC2=O)=O)=O)=O)C(Cl)=C1C(C3=CC4=C(NC(C)(C)CC4C)C(S(=O)([O-])=O)=C3O5)=C6C5=C(S(=O)([O-])=O)C7=[NH+]C(C)(C)CC(C)C7=C6.CC[NH+](CC)CC |
上方提供的技术数据仅供参考。批次相关数据请参见分析证书。
Tocris products are intended for laboratory research use only, unless stated otherwise.
参考文献是支持产品生物活性的出版物。
Hone et al (2010) Alexa Fluor 546-ArIB[V11L;V16A] is a potent ligand for selectively labeling α 7 nicotinic acetylcholine receptors. J.Neurochem. 114 994 PMID: 20492354
Iwata et al (2005) Increased susceptibility of cytoplasmic over nuclear polyglutamine aggregates to autophagic degradation. Proc.Natl.Acad.Sci.USA. 102 13135 PMID: 16141322
Panchuk-Voloshina et al (1999) Alexa dyes, a series of new fluorescent dyes that yield exceptionally bright, photostable conjugates. J.Histochem.Cytochem. 47 1179 PMID: 10449539
关键词: TFAX 546, SE, TFAX 546, SE supplier, TFAX546,, SE, TFAX546SE, Amine, reactive, yellow, fluorescent, dye, microscopy, flow, cytometry, CAS, 477876-66-7, TFAX, Dyes, Flow, Cytometry, 6623, Tocris Bioscience
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