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Description: Oct3/4 inducer; induces expression of pluripotent-associated genes
Chemical Name: Ethyl 2-[(4-chlorophenyl)amino]-4-thiazolecarboxylate
Purity: ≥98% (HPLC)
Biological Activity for O4I2
O4I2 is an Oct3/4 inducer; promotes Oct3/4 expression and stabilizes the Oct3/4 protein in HEK293 cells. Induces expression of pluripotent-associated genes Lin28, Sox2 and Nanog, and suppresses Rex1. Induces 15-fold increase in Oct3/4 expression in human primary fibroblasts.
Compound Libraries for O4I2
O4I2 is also offered as part of the Tocriscreen Stem Cell Library. Find out more about compound libraries available from Tocris.
Technical Data for O4I2
| M. Wt | 282.75 |
| Formula | C12H11ClN2O2S |
| Storage | Store at -20°C |
| Purity | ≥98% (HPLC) |
| CAS Number | 165682-93-9 |
| PubChem ID | 53428670 |
| InChI Key | ULUBAPWNHROTEU-UHFFFAOYSA-N |
| Smiles | ClC(C=C2)=CC=C2NC1=NC(C(OCC)=O)=CS1 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for O4I2
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 28.27 | 100 | |
| ethanol | 14.14 | 50 |
Preparing Stock Solutions for O4I2
The following data is based on the product molecular weight 282.75. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 3.54 mL | 17.68 mL | 35.37 mL |
| 5 mM | 0.71 mL | 3.54 mL | 7.07 mL |
| 10 mM | 0.35 mL | 1.77 mL | 3.54 mL |
| 50 mM | 0.07 mL | 0.35 mL | 0.71 mL |
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Product Datasheets for O4I2
References for O4I2
References are publications that support the biological activity of the product.
Cheng et al (2015) Ethyl 2-((4-Chlorophenyl)amino)thiazole-4-carboxylate and derivatives are potent inducers of Oct3/4. J.Med.Chem. 58 5742 PMID: 26143659
If you know of a relevant reference for O4I2, please let us know.
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Citations for O4I2
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Literature in this Area
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Stem Cell Research Product Guide
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