DNA Ligases

DNA ligases play an integral role in DNA repair and replication through catalyzing the formation of phosphodiester bonds. Two classes of DNA ligase have been identified: ATP-dependent (EC 6.5.1.1), and NAD+-dependent (EC 6.5.1.2).

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DNA ligases play an integral role in DNA repair and replication through catalyzing the formation of phosphodiester bonds. Two types of DNA ligase have been identified: ATP-dependent DNA ligases (EC 6.5.1.1), and NAD+-dependent DNA ligases (EC 6.5.1.2).

The ATP-dependent DNA ligases are divided into four classes: DNA ligase I, II, III and IV. DNA ligase I links Okazaki fragments to form a continuous strand of DNA; DNA ligase II is an alternatively spliced form of DNA ligase III, found only in non-dividing cells; DNA ligase III is involved in base excision repair; and DNA ligase IV is involved in the repair of DNA double-strand breaks by non-homologous end joining (NHEJ).

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