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Description: Potent and selective PDE4B inhibitor
Chemical Name: 2-[4-[[2-(5-Chloro-2-thienyl)-5-ethyl-6-methyl-pyrimidin-4-yl]amino]phenyl]acetic acid
Purity: ≥98% (HPLC)
Biological Activity for A 33
A 33 is a potent and selective PDE4B inhibitor (IC50 = 15 nM); exhibits >100-fold selectivity for PDE4B over 4D. Regulates cAMP in inflammatory cells. Inhibits LPS-induced TNF-α production and neutrophil accumulation in mouse lungs. Reduces microglial and neutrophil activation, and improves behavioral deficits in a traumatic brain injury model in vivo. Also exhibits antidepressant-like effects. Orally bioavailable.
Technical Data for A 33
| M. Wt | 387.88 |
| Formula | C19H18ClN3O2S |
| Storage | Store at -20°C |
| Purity | ≥98% (HPLC) |
| CAS Number | 915082-52-9 |
| PubChem ID | 15951010 |
| InChI Key | FDVSPBLZPJMXFV-UHFFFAOYSA-N |
| Smiles | CCC1=C(N=C(C2=CC=C(S2)Cl)N=C1NC3=CC=C(C=C3)CC(O)=O)C |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for A 33
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 38.79 | 100 |
Preparing Stock Solutions for A 33
The following data is based on the product molecular weight 387.88. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 2.58 mL | 12.89 mL | 25.78 mL |
| 5 mM | 0.52 mL | 2.58 mL | 5.16 mL |
| 10 mM | 0.26 mL | 1.29 mL | 2.58 mL |
| 50 mM | 0.05 mL | 0.26 mL | 0.52 mL |
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Product Datasheets for A 33
References for A 33
References are publications that support the biological activity of the product.
Naganuma et al (2009) Discovery of selective PDE4B inhibitors. Bioorg.Med.Chem.Lett. 19 3174 PMID: 19447034
Zhang et al (2017) Comparison of the pharmacological profiles of selective PDE4B and PDE4D inhibitors in the central nervous system. Sci.Rep. 7 40115 PMID: 28054669
Wilson et al (2017) Therapeutic benefits of phosphodiesterase 4B inhibition after traumatic brain injury. PLoS One 12 e0178013 PMID: 28542295
If you know of a relevant reference for A 33, please let us know.
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Keywords: A 33, A 33 supplier, A33, potent, selective, PDE4B, phosphodiesterase, inhibitors, inhibits, orally, bioavailable, anti-inflammatory, antidepressant, TBI, traumatic, brain, injury, Phosphodiesterases, 6313, Tocris Bioscience
1 Citation for A 33
Citations are publications that use Tocris products. Selected citations for A 33 include:
Eyal et al (2022) PDE4B Is a Homeostatic Regulator of Cyclic AMP in Dendritic Cells. Front Pharmacol 13 833832 PMID: 35387344
Do you know of a great paper that uses A 33 from Tocris? Please let us know.
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