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cis-Ned 19

Cat. No. 3953 2 Citations Submit a Review Datasheet / COA / SDS
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Description: NAADP antagonist; inhibits Ca2+ release
Chemical Name: (1S,3S)-1-[3-[[4-(2-Fluorophenyl)piperazin-1-yl]methyl]-4-methoxyphenyl]-2,3,4,9-tetrahydro-1H-pyrido[3,4-b]indole-3-carboxylic acid
Purity: ≥97% (HPLC)
Datasheet
Citations (2)
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Biological Activity Technical Data Solubility Calculators Datasheets References

Biological Activity for cis-Ned 19

cis-Ned 19 is an irreversible nicotinic acid adenine dinucleotide phosphate (NAADP) antagonist; inhibits Ca2+ release (IC50 = 800 nM) and [32P]NAADP binding (IC50 = 15 μM). Stereoisomer of trans-Ned 19. Fluorescently labels NAADP receptors in intact cells (excitation 351 and 365 nm using an ultraviolet Argon laser).

Isomer of trans-Ned 19.

Optical Data for cis-Ned 19

λabs 368 nm
λem 425 nm
Closest Laser line 355 nm
Cell Permeable Yes
Application Confocal Microscopy

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Technical Data for cis-Ned 19

M. Wt 514.59
Formula C30H31FN4O3
Storage Store at -20°C
Purity ≥97% (HPLC)
CAS Number 1137264-00-6
PubChem ID 1427626
InChI Key FUHCEERDBRGPQZ-LSYYVWMOSA-N
Smiles FC1=CC=CC=C1N(CC6)CCN6CC2=C[C@@]([C@H]3C4=C(C(C=CC=C5)=C5N4)C[C@@H]([C@@](O)=O)N3)=CC=C2OC

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for cis-Ned 19

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 51.46 100

Preparing Stock Solutions for cis-Ned 19

The following data is based on the product molecular weight 514.59. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.94 mL 9.72 mL 19.43 mL
5 mM 0.39 mL 1.94 mL 3.89 mL
10 mM 0.19 mL 0.97 mL 1.94 mL
50 mM 0.04 mL 0.19 mL 0.39 mL

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References for cis-Ned 19

References are publications that support the biological activity of the product.

Naylor et al (2009) Identification of a chemical probe for NAADP by virtual screening. Nat.Chem.Biol. 5 220 PMID: 19234453

If you know of a relevant reference for cis-Ned 19, please let us know.

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Keywords: cis-Ned 19, cis-Ned 19 supplier, cis-Ned19, NAADP, nicotinic, acid, adenine, dinucleotide, phosphate, antagonists, calcium, signaling, signalling, inhibitors, inhibits, fluorescent, probes, ned19, General, Calcium, Signaling, Agents, 3953, Tocris Bioscience

2 Citations for cis-Ned 19

Citations are publications that use Tocris products. Selected citations for cis-Ned 19 include:

Favia et al (2014) VEGF-induced neoangiogenesis is mediated by NAADP and two-pore channel-2-dependent Ca2+ signaling. Proc Natl Acad Sci U S A 111 E4706 PMID: 25331892

Gerasimenko et al (2015) Both RyRs and TPCs are required for NAADP-induced intracellular Ca2+ release. Sci Rep 58 237 PMID: 26100948

Do you know of a great paper that uses cis-Ned 19 from Tocris? Please let us know.

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