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Discontinued Product
3953 has been discontinued.
View all General Calcium Signaling Agents products.
Description: NAADP antagonist; inhibits Ca2+ release
Chemical Name: (1S,3S)-1-[3-[[4-(2-Fluorophenyl)piperazin-1-yl]methyl]-4-methoxyphenyl]-2,3,4,9-tetrahydro-1H-pyrido[3,4-b]indole-3-carboxylic acid
Purity: ≥97% (HPLC)
Biological Activity for cis-Ned 19
cis-Ned 19 is an irreversible nicotinic acid adenine dinucleotide phosphate (NAADP) antagonist; inhibits Ca2+ release (IC50 = 800 nM) and [32P]NAADP binding (IC50 = 15 μM). Stereoisomer of trans-Ned 19. Fluorescently labels NAADP receptors in intact cells (excitation 351 and 365 nm using an ultraviolet Argon laser).
Isomer of trans-Ned 19.
Optical Data for cis-Ned 19
| λabs | 368 nm |
|---|---|
| λem | 425 nm |
| Closest Laser line | 355 nm |
| Cell Permeable | Yes |
| Application | Confocal Microscopy |
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectral Viewer
Technical Data for cis-Ned 19
| M. Wt | 514.59 |
| Formula | C30H31FN4O3 |
| Storage | Store at -20°C |
| Purity | ≥97% (HPLC) |
| CAS Number | 1137264-00-6 |
| PubChem ID | 1427626 |
| InChI Key | FUHCEERDBRGPQZ-LSYYVWMOSA-N |
| Smiles | FC1=CC=CC=C1N(CC6)CCN6CC2=C[C@@]([C@H]3C4=C(C(C=CC=C5)=C5N4)C[C@@H]([C@@](O)=O)N3)=CC=C2OC |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Product Datasheets for cis-Ned 19
References for cis-Ned 19
References are publications that support the biological activity of the product.
Naylor et al (2009) Identification of a chemical probe for NAADP by virtual screening. Nat.Chem.Biol. 5 220 PMID: 19234453
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Keywords: cis-Ned 19, cis-Ned 19 supplier, cis-Ned19, NAADP, nicotinic, acid, adenine, dinucleotide, phosphate, antagonists, calcium, signaling, signalling, inhibitors, inhibits, fluorescent, probes, ned19, General, Calcium, Signaling, Agents, 3953, Tocris Bioscience
2 Citations for cis-Ned 19
Citations are publications that use Tocris products. Selected citations for cis-Ned 19 include:
Gerasimenko et al (2015) Both RyRs and TPCs are required for NAADP-induced intracellular Ca2+ release. Sci Rep 58 237 PMID: 26100948
Favia et al (2014) VEGF-induced neoangiogenesis is mediated by NAADP and two-pore channel-2-dependent Ca2+ signaling. Proc Natl Acad Sci U S A 111 E4706 PMID: 25331892
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