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Submit ReviewCK 666
Description: Arp2/3 inhibitor; inhibits actin polymerization
Chemical Name: 2-Fluoro-N-[2-(2-methyl-1H-indol-3-yl)ethyl]benzamide
Purity: ≥99% (HPLC)
Biological Activity for CK 666
CK 666 is an inhibitor of the Arp2/3 complex; inhibits actin polymerization (IC50 = 4 μM).
Technical Data for CK 666
| M. Wt | 296.34 |
| Formula | C18H17FN2O |
| Storage | Store at -20°C |
| Purity | ≥99% (HPLC) |
| CAS Number | 442633-00-3 |
| PubChem ID | 589075 |
| InChI Key | UXRKUKRXVWJFER-UHFFFAOYSA-N |
| Smiles | FC1=CC=CC=C1C(NCCC2=C(C)NC3=C2C=CC=C3)=O |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for CK 666
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 29.63 | 100 | |
| ethanol | 29.63 | 100 |
Preparing Stock Solutions for CK 666
The following data is based on the product molecular weight 296.34. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 3.37 mL | 16.87 mL | 33.75 mL |
| 5 mM | 0.67 mL | 3.37 mL | 6.75 mL |
| 10 mM | 0.34 mL | 1.69 mL | 3.37 mL |
| 50 mM | 0.07 mL | 0.34 mL | 0.67 mL |
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Product Datasheets for CK 666
References for CK 666
References are publications that support the biological activity of the product.
Nolen et al (2009) Characterization of two classes of small molecule inhibitors of Arp2/3 complex. Nature 460 1031 PMID: 19648907
Han and Nolen (2009) X-ray crystal structures and molecular mechanism of improved Arp2/3 complex inhibitors. 1751 ACSB 2009 Abstracts. American Society for Cel
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Keywords: CK 666, CK 666 supplier, CK666, arp2/3, actin, polymerization, inhibitors, inhibits, actin-related, proteins, arp2, arp3, Actin, 3950, Tocris Bioscience
26 Citations for CK 666
Citations are publications that use Tocris products. Selected citations for CK 666 include:
Charles B et al (2019) Cytoskeletal polarization and cytokinetic signaling drives polar lobe formation in spiralian embryos. Dev Biol 456 201-211 PMID: 31479647
Matthieu et al (2019) Macropinocytosis Overcomes Directional Bias in Dendritic Cells Due to Hydraulic Resistance and Facilitates Space Exploration. Dev Cell 49 171-188.e5 PMID: 30982662
Bharadwaj (2017) αV-class integrins exert dual roles on α5β1 integrins to strengthen adhesion to fibronectin. Nat Commun 8 14348 PMID: 28128308
Daniel L et al (2022) Nuclear F-actin and Lamin A antagonistically modulate nuclear shape. J Cell Sci 135 PMID: 35665815
Raymond P et al (2022) Identification of a pharmacological approach to reduce ACE2 expression and development of an in vitro COVID-19 viral entry model. J Virus Erad 8 100307 PMID: 36514715
Roesler et al (2019) Myosin XVI Regulates Actin Cytoskeleton Dynamics in Dendritic Spines of Purkinje Cells and Affects Presynaptic Organization. Front Cell Neurosci 13 330 PMID: 31474830
Panousopoulou et al (2016) Epiboly generates the epidermal basal monolayer and spreads the nascent mammalian skin to enclose the embryonic body. J Cell Sci 129 1915 PMID: 26989131
Jacob et al (2023) Plasma membrane topography governs the 3D dynamic localization of IgM B cell antigen receptor clusters. EMBO J 42 e112030 PMID: 36594262
Philippe et al (2020) Wiskott-Aldrich syndrome protein restricts cGAS/STING activation by dsDNA immune complexes. JCI Insight 5 PMID: 32721945
William M et al (2020) Mechanism of Long-Range Chromosome Motion Triggered by Gene Activation. Dev Cell 52 309-320.e5 PMID: 31902656
Yamagashi et al (2018) Use of CK-548 and CK-869 as Arp2/3 complex inhibitors directly suppresses microtubule assembly both in vitro and in vivo. Biochem Biophys Res Commun 496 834 PMID: 29395083
Casper C et al (2018) Activity-Dependent Actin Remodeling at the Base of Dendritic Spines Promotes Microtubule Entry. Curr Biol 28 2081-2093.e6 PMID: 29910073
Visar et al (2020) Wound Healing Coordinates Actin Architectures to Regulate Mechanical Work. Nat Phys 15 696-705 PMID: 31897085
Charles B et al (2020) Rac and Arp2/3-Nucleated Actin Networks Antagonize Rho During Mitotic and Meiotic Cleavages. Front Cell Dev Biol 8 591141 PMID: 33282870
Sepúlveda-Ramírez et al (2018) Cdc42 controls primary mesenchyme cell morphogenesis in the sea urchin embryo. Dev Biol 437 140 PMID: 29555242
Han et al (2016) Macrophages redirect phagocytosis by non-professional phagocytes and influence inflammation Nature 539 570 PMID: 27820945
Dalton and Carroll (2013) Biased inheritance of mitochondria during asymmetric cell division in the mouse oocyte. J Cell Sci 126 2955 PMID: 23659999
Kurt I et al (2021) Single-cell resolved imaging reveals intra-tumor heterogeneity in glycolysis, transitions between metabolic states, and their regulatory mechanisms. Cell Rep 34 108750 PMID: 33596424
Jeremy S et al (2021) Melanoma cells adopt features of both mesenchymal and amoeboid migration within confining channels. Sci Rep 11 17804 PMID: 34493759
Logue et al (2018) c-Src activity is differentially required by cancer cell motility modes. Oncogene 37 2104 PMID: 29379163
Tabdanov et al (2018) Bimodal sensing of guidance cues in mechanically distinct microenvironments. Nat Commun 9 4891 PMID: 30459308
Tabdanov et al (2018) Microtubule-Actomyosin Mechanical Cooperation during Contact Guidance Sensing. Cell Rep 25 328 PMID: 30304674
Tornavaca et al (2015) ZO-1 controls endothelial adherens junctions, cell-cell tension, angiogenesis, and barrier formation. Proc Natl Acad Sci U S A 208 821 PMID: 25753039
Moreau et al (2015) Signal strength regulates antigen-mediated T-cell deceleration by distinct mechanisms to promote local exploration or arrest. Nat Commun 112 12151 PMID: 26371316
Sorce et al (2015) Mitotic cells contract actomyosin cortex and generate pressure to round against or escape epithelial confinement. Cell Calcium 6 8872 PMID: 26602832
Basu et al (2016) Arp2/3 and VASP Are Essential for Fear Memory Formation in Lateral Amygdala. Eneuro 3 PMID: 27957528
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