Submit a Review & Earn an Amazon Gift Card
You can now submit reviews for your favorite Tocris products. Your review will help other researchers decide on the best products for their research. Why not submit a review today?!
Submit ReviewCRID3 sodium salt
Description: Potent NLRP3 inflammasome inhibitor; inhibits IL-1β production
Alternative Names: CP-456773,MCC 950
Chemical Name: N-[[(1,2,3,5,6,7-Hexahydro-s-indacen-4-yl)amino]carbonyl]-4-(1-hydroxy-1-methylethyl)-2-furansulfonamide sodium salt
Purity: ≥98% (HPLC)
Biological Activity for CRID3 sodium salt
CRID3 sodium salt is a potent NLRP3 inflammasome inhibitor; closes the active conformation of NLRP3 to the inactive state. Directly interacts with the Walker B motif within the NLRP3 NACHT domain. Inhibits IL-1β, IL-18 and IL-1α production (IC50 values are 7.2, 10.3 and 12-18 nM, respectively). Selective for NLRP3 over NLRC4 inflammasome and Toll-like receptor signaling. Reduces severity of experimental autoimmune encephalomyelitis, skin inflammation and airway inflammation in mice. Also glutathione S-transferase omega 1 inhibitor. Orally bioavailable.
Licensing Information
Sold for research purposes under agreement from Pfizer Inc
Technical Data for CRID3 sodium salt
| M. Wt | 426.46 |
| Formula | C20H23N2NaO5S |
| Storage | Store at +4°C |
| Purity | ≥98% (HPLC) |
| CAS Number | 256373-96-3 |
| PubChem ID | 91826093 |
| InChI Key | LFQQNXFKPNZRFT-UHFFFAOYSA-M |
| Smiles | O=C(N(S(C4=CC(C(C)(O)C)=CO4)(=O)=O)[Na])NC1=C3C(CCC3)=CC2=C1CCC2 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for CRID3 sodium salt
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| water | 42.65 | 100 | |
| DMSO | 42.65 | 100 |
Preparing Stock Solutions for CRID3 sodium salt
The following data is based on the product molecular weight 426.46. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 2.34 mL | 11.72 mL | 23.45 mL |
| 5 mM | 0.47 mL | 2.34 mL | 4.69 mL |
| 10 mM | 0.23 mL | 1.17 mL | 2.34 mL |
| 50 mM | 0.05 mL | 0.23 mL | 0.47 mL |
Molarity Calculator
Molarity Calculator
Calculate the mass, volume, or concentration required for a solution.
Reconstitution Calculator
Reconstitution Calculator
Dilution Calculator
Dilution Calculator
Calculate the dilution required to prepare a stock solution.
Product Datasheets for CRID3 sodium salt
References for CRID3 sodium salt
References are publications that support the biological activity of the product.
Laliberte et al (2003) Glutathione S-transferase omega 1-1 is a target of cytokine release inhibitory drugs and may be responsible for their effect on interleukin-1β posttranslational processing. J.Biol.Chem. 278 16567 PMID: 12624100
Coll et al (2015) A small-molecule inhibitor of the NLRP3 inflammasome for the treatment of inflammatory diseases. Nat.Med. 21 248 PMID: 25686105
Gordon et al (2018) Inflammasome inhibition prevents a-synuclein pathology and dopaminergic neurodegeneration in mice. Sci.Transl.Med. 10 eaah4066 PMID: 30381407
Tapia-Abellán et al (2019) MCC950 closes the active conformation of NLRP3 to an inactive state. Nat.Chem.Biol. 15 560 PMID: 31086329
Coll et al (2019) MCC950 directly targets the NLRP3 ATP-hydrolysis motif for inflammasome inhibition. Nat.Chem.Biol. 15 556 PMID: 31086327
If you know of a relevant reference for CRID3 sodium salt, please let us know.
View Related Products by Target
Keywords: CRID3 sodium salt, CRID3 sodium salt supplier, CP456773, cytokine, release, inhibitory, drug, CRID3, Glutathione, S-transferase, omega, 1, GSTO1, inhibitors, inhibits, MCC950, nod-like, receptors, NLRP3, inflammasome, CP-456773, MCC, 950, Other, Transferases, Cytokines, Inflammasomes, 5479, Tocris Bioscience
10 Citations for CRID3 sodium salt
Citations are publications that use Tocris products. Selected citations for CRID3 sodium salt include:
David A et al (2022) Interleukin 38 alleviates aortic valve calcification by inhibition of NLRP3. Proc Natl Acad Sci U S A 119 e2202577119 PMID: 36037361
Flóra et al (2022) Maternal P2X7 receptor inhibition prevents autism-like phenotype in male mouse offspring through the NLRP3-IL-1β pathway. Brain Behav Immun 101 318-332 PMID: 35065198
Goddard et al (2019) Enteropathogenic Escherichia coli Stimulates Effector-Driven Rapid Caspase-4 Activation in Human Macrophages. Cell Rep 27 1008 PMID: 31018119
Oliver T et al (2020) CARD8 inflammasome activation triggers pyroptosis in human T cells. EMBO J 39 e105071 PMID: 32840892
Minqi et al (2020) Eldecalcitol Inhibits LPS-Induced NLRP3 Inflammasome-Dependent Pyroptosis in Human Gingival Fibroblasts by Activating the Nrf2/HO-1 Signaling Pathway. Drug Des Devel Ther 14 4901-4913 PMID: 33223823
Nagesh et al (2020) Inhibition of Amyloid-Beta Production, Associated Neuroinflammation, and Histone Deacetylase 2-Mediated Epigenetic Modifications Prevent Neuropathology in Alzheimer's Disease in vitro Model. Front Aging Neurosci 11 342 PMID: 32009938
Nitya et al (2020) Genetic and pharmacological inhibition of inflammasomes reduces the survival of Mycobacterium tuberculosis strains in macrophages. Sci Rep 10 3709 PMID: 32111888
Chauhan et al (2018) BAX/BAK-Induced Apoptosis Results in Caspase-8-Dependent IL-1β Maturation in Macrophages. Cell Rep 25 2354 PMID: 30485805
Eduardo et al (2021) An in vitro study of ApxI from Actinobacillus pleuropneumoniae serotype 10 and induction of NLRP3 inflammasome-dependent cell death. Vet Rec Open 8 e20 PMID: 34631111
Catherine B et al (2021) Inhibition of the NLRP3 inflammasome by HSP90 inhibitors. Immunology 162 84-91 PMID: 32954500
Do you know of a great paper that uses CRID3 sodium salt from Tocris? Please let us know.
Reviews for CRID3 sodium salt
There are currently no reviews for this product. Be the first to review CRID3 sodium salt and earn rewards!
Have you used CRID3 sodium salt?
Submit a review and receive an Amazon gift card.
$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Literature in this Area
Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!
*Please note that Tocris will only send literature to established scientific business / institute addresses.
Rheumatoid Arthritis Poster
Rheumatoid arthritis (RA) is a chronic destructive inflammatory autoimmune disease that results from a breakdown in immune tolerance, for reasons that are as yet unknown. This poster summarizes the pathology of RA and the inflammatory processes involved, as well as describing some of the epigenetic modifications associated with the disease and the potential for targeting these changes in the discovery of new treatments.