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Submit ReviewCytochalasin B
Description: Inhibitor of actin polymerization
Chemical Name: (3E,5R,9R,11E,12aS,13S,15S,15aS,16S,18aS)-6,7,8,9,10,12a,13,14,15,15a,16,17-Dodecahydro-5,13-dihydroxy-9,15-dimethyl-14-methylene-16-(phenylmethyl)-2H-oxacyclotetradecino[2,3-d]isoindole-2,18(5H)-dione
Purity: ≥98% (HPLC)
Biological Activity for Cytochalasin B
Cytochalasin B is an inhibitor of actin polymerization. Inhibits cell division, migration and glucose transport. Causes G2/M cell cycle arrest and apoptosis in HCT-116 colorectal carcinoma cells. Cell permeable.
Technical Data for Cytochalasin B
| M. Wt | 479.61 |
| Formula | C29H37NO5 |
| Storage | Store at -20°C |
| Purity | ≥98% (HPLC) |
| CAS Number | 14930-96-2 |
| PubChem ID | 5311281 |
| InChI Key | GBOGMAARMMDZGR-TYHYBEHESA-N |
| Smiles | C=C([C@H]1C)[C@@H](O)[C@]([C@@]2(O3)[C@]1([H])[C@H](CC4=CC=CC=C4)NC2=O)([H])/C=C/C[C@H](C)CCC[C@@H](O)/C=C/C3=O |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for Cytochalasin B
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 4.8 | 10 with gentle warming |
Preparing Stock Solutions for Cytochalasin B
The following data is based on the product molecular weight 479.61. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.1 mM | 20.85 mL | 104.25 mL | 208.5 mL |
| 0.5 mM | 4.17 mL | 20.85 mL | 41.7 mL |
| 1 mM | 2.09 mL | 10.43 mL | 20.85 mL |
| 5 mM | 0.42 mL | 2.09 mL | 4.17 mL |
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Product Datasheets for Cytochalasin B
References for Cytochalasin B
References are publications that support the biological activity of the product.
Theodoropoulos et al (1994) Cytochalasin B may shorten actin filaments by a mechanism independent of barbed end capping. Biochem.Pharmacol. 47 1875 PMID: 8204105
Whitesell et al (2005) Compartmentalization of transport and phosphorylation of glucose in a hepatoma cell line. Biochem.J. 386 245 PMID: 15473866
Buldak et al (2014) Changes in subcellular localization of visfatin in human colorectal HCT-116 carcinoma cell line after cytochalasin B treatment. Eur.J.Histochem. 58 2408 PMID: 25308845
If you know of a relevant reference for Cytochalasin B, please let us know.
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Keywords: Cytochalasin B, Cytochalasin B supplier, inhibitors, inhibits, actin, polymerization, polymerisation, cell, permeable, division, migration, cycle, arrest, cytokinesis, Actin, 5474, Tocris Bioscience
7 Citations for Cytochalasin B
Citations are publications that use Tocris products. Selected citations for Cytochalasin B include:
David Ian et al (2022) Development of reconstructed intestinal micronucleus cytome (RICyt) assay in 3D human gut model for genotoxicity assessment of orally ingested substances. Arch Toxicol 96 1455-1471 PMID: 35226136
Ingrid et al (2022) WASp triggers mechanosensitive actin patches to facilitate immune cell migration in dense tissues. Dev Cell 57 47-62.e9 PMID: 34919802
Zheng et al (2019) A High-Content Screen Identifies TPP1 and Aurora B as Regulators of Axonal Mitochondrial Transport. Cell Rep 28 3224-3237.e5 PMID: 31533043
Bernier et al (2019) Nanoscale Surveillance of the Brain by Microglia via cAMP-Regulated Filopodia. Cell Rep 27 2895 PMID: 31167136
Stefanie M et al (2023) Deficits in mitochondrial TCA cycle and OXPHOS precede rod photoreceptor degeneration during chronic HIF activation. Mol Neurodegener 18 15 PMID: 36882871
Diaz-Garcia et al (2017) Neuronal Stimulation Triggers Neuronal Glycolysis and Not Lactate Uptake. Cell Metab 26 361 PMID: 28768175
Maria F et al (2021) ADF and cofilin-1 collaborate to promote cortical actin flow and the leader bleb-based migration of confined cells. Elife 10 PMID: 34169836
Do you know of a great paper that uses Cytochalasin B from Tocris? Please let us know.
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