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Submit ReviewdTAG Janelia Fluor® 525 New
Description: Fluorogenic srTAG probe for live cell imaging of FBKP12 (F36V/L) fusion proteins
Chemical Name: 2-(3-(3,3-Difluoroazetidin-1-ium-1-ylidene)-6-(3,3-difluoroazetidin-1-yl)-3H-xanthen-9-yl)-4-((2-(2-(3-((R)-3-(3,4-dimethoxyphenyl)-1-(((S)-1-((S)-2-(3,4,5-trimethoxyphenyl)butanoyl)piperidine-2-carbonyl)oxy)propyl)phenoxy)acetamido)ethyl)carbamoyl)benzoate
Purity: ≥95% (HPLC)
Biological Activity for dTAG Janelia Fluor® 525
Key Information: dTAG Janelia Fluor® 525 is a fluorogenic srTAG probe for live cell imaging of FKBP12F36V/L fusion proteins.
Application: Suitable for confocal microscopy and Super Resolution Microscopy (SRM) including STED. dTAG Janelia Fluor® 525 is cell permeable.
Properties and Photophysical Data: In FRAP (fluorescence recovery after photobleaching) experiment, the highest maximum recovery ratio (73%) was shown when U2OS cells expressing FKBPF36L were incubated with 10 μM dTAG Janelia Fluor® 525; excitation and emission maxima (λ) are 530 nm and 560 nm, respectively; quantum yield = 0.47; extinction coefficient = 82,800 M-1cm-1 (in 4% TFA).
It is recommended to use a working concentration of 10 μM in live cell imaging application.
Janelia Fluor is a registered trademark of Howard Hughes Medical Institute.
Optical Data for dTAG Janelia Fluor® 525
| Emission Color | Yellow |
|---|---|
| Brightness | |
| λabs | 530 nm |
| λem | 560 nm |
| Extinction Coefficient (ε) | 82800 M-1cm-1 |
| Quantum Yield (φ) | 0.47 |
| Closest Laser line | 488 nm |
| Cell Permeable | Yes |
| Application | Confocal Microscopy, Super Resolution Microscopy |
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Technical Data for dTAG Janelia Fluor® 525
| M. Wt | 1244.3 |
| Formula | C67H69F4N5O14 |
| Storage | Store at -20°C |
| Purity | ≥95% (HPLC) |
| InChI Key | ZSEJCFOGOGMEPJ-FUJQAITOSA-N |
| Smiles | O=C([C@@H]1CCCCN1C([C@H](C2=CC(OC)=C(OC)C(OC)=C2)CC)=O)O[C@@H](C3=CC(OCC(NCCNC(C4=CC=C(C([O-])=O)C(C(C5=CC=C(N6CC(F)(F)C6)C=C5O7)=C(C=C/8)C7=CC8=[N+]9CC(F)(F)C\9)=C4)=O)=O)=CC=C3)CCC%10=CC(OC)=C(OC)C=C%10 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for dTAG Janelia Fluor® 525
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 12.44 | 10 |
Preparing Stock Solutions for dTAG Janelia Fluor® 525
The following data is based on the product molecular weight 1244.3. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.1 mM | 8.04 mL | 40.18 mL | 80.37 mL |
| 0.5 mM | 1.61 mL | 8.04 mL | 16.07 mL |
| 1 mM | 0.8 mL | 4.02 mL | 8.04 mL |
| 5 mM | 0.16 mL | 0.8 mL | 1.61 mL |
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Product Datasheets for dTAG Janelia Fluor® 525
References for dTAG Janelia Fluor® 525
References are publications that support the biological activity of the product.
Du et al (2023) Self-renewable tag for photostable fluorescence imaging of proteins. J.Am.Chem.Soc. 145 18968 PMID: 37596976
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