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Submit ReviewdTAG Janelia Fluor® 635
Description: Fluorogenic srTAG probe for live cell imaging of FBKP12 (F36V/L) labeled proteins
Chemical Name: 4-((2-(2-(3-((R)-3-(3,4-Dimethoxyphenyl)-1-(((S)-1-((S)-2-(3,4,5-trimethoxyphenyl)butanoyl)piperidine-2-carbonyl)oxy)propyl)phenoxy)acetamido)ethyl)carbamoyl)-2-(3-(3-fluoroazetidin-1-ium-1-ylidene)-7-(3-fluoroazetidin-1-yl)-5,5-dimethyl-3,5-dihydrodibenzo[b,e]silin-10-yl)benzoate
Purity: ≥95% (HPLC)
Biological Activity for dTAG Janelia Fluor® 635
Key Information: dTAG Janelia Fluor® 635 is a fluorogenic srTAG probe for live cell imaging of FKBP12F36V/L fusion proteins.
Application: Suitable for confocal microscopy and Super Resolution Microscopy (SRM) including STED. dTAG Janelia Fluor® 635 is cell permeable.
Properties and Photophysical Data: In FRAP (fluorescence recovery after photobleaching) experiment, the highest maximum recovery ratio (85%) was shown when U2OS cells expressing FKBPF36L were incubated with 10 μM dTAG Janelia Fluor® 635; excitation and emission maxima (λ) are 640 nm and 665 nm, respectively; quantum yield = 0.41; extinction coefficient = 75,000 M-1cm-1 (in 4% TFA).
Janelia Fluor is a registered trademark of Howard Hughes Medical Institute.
Scientific Data
Fluorescence Imaging
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Application of dTAG Janelia Fluor® 635. HEK293 cells were incubated for 1 hour with 2.5 μM dTAG Janelia Fluor® 635 (red) and Hoechst (blue), without (left) or with (right) endogenous protein of interest labeled with dTAG/FKBP12F36V. Fluorescent microscopy images kindly provided by Yongli Shan, Vincinitas Therapeutics.
Fluorescence Imaging
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Application of dTAG Janelia Fluor® 635. HEK293 cells were incubated for 1 hour with different concentrations of dTAG Janelia Fluor® 635 (red) and Hoechst (blue), without (top) or with (bottom) endogenous protein of interest labeled with dTAG/FKBP12F36V. Images captured by high content imaging were kindly provided by Yongli Shan, Vincinitas Therapeutics.
Licensing Information
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus
Optical Data for dTAG Janelia Fluor® 635
| Emission Color | Red |
|---|---|
| Brightness | |
| λabs | 640 nm |
| λem | 665 nm |
| Extinction Coefficient (ε) | 75000 M-1cm-1 |
| Quantum Yield (φ) | 0.41 |
| Closest Laser line | 635 nm |
| Cell Permeable | Yes |
| Application | Confocal Microscopy, Super Resolution Microscopy |
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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Technical Data for dTAG Janelia Fluor® 635
| M. Wt | 1250.18 |
| Formula | C69H77F2N5O13Si |
| Storage | Store at -20°C |
| Purity | ≥95% (HPLC) |
| InChI Key | UPUINLLKPHUNRA-QXDPIUJBSA-N |
| Smiles | O=C([C@@H]1CCCCN1C([C@H](C2=CC(OC)=C(OC)C(OC)=C2)CC)=O)O[C@@H](C3=CC(OCC(NCCNC(C4=CC=C(C([O-])=O)C(C(C5=CC=C(N6CC(F)C6)C=C5[Si]7(C)C)=C(C=C/8)C7=CC8=[N+]9CC(F)C\9)=C4)=O)=O)=CC=C3)CCC%10=CC(OC)=C(OC)C=C%10 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for dTAG Janelia Fluor® 635
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 12.5 | 10 |
Preparing Stock Solutions for dTAG Janelia Fluor® 635
The following data is based on the product molecular weight 1250.18. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.1 mM | 8 mL | 39.99 mL | 79.99 mL |
| 0.5 mM | 1.6 mL | 8 mL | 16 mL |
| 1 mM | 0.8 mL | 4 mL | 8 mL |
| 5 mM | 0.16 mL | 0.8 mL | 1.6 mL |
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Product Datasheets for dTAG Janelia Fluor® 635
References for dTAG Janelia Fluor® 635
References are publications that support the biological activity of the product.
Du et al (2024) Self-renewable tag for photostable fluorescence imaging of proteins. J.Am.Chem.Soc. 145 18968 PMID: 37596976
If you know of a relevant reference for dTAG Janelia Fluor® 635, please let us know.
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Keywords: dTAG Janelia Fluor® 635, dTAG Janelia Fluor® 635 supplier, labeling, probe, FKBP12(F36V), dTAG, fluorescein, fluorescence, super, resolution, microscopy, TAG, Other, Fluorescent, FRAP, SRM, STED, permeable, ZCD, 1, ZCD-1, Probes, Degradation, Platform, Self-labeling, Tags, 8101, Tocris Bioscience
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