Submit a Review & Earn an Amazon Gift Card
You can now submit reviews for your favorite Tocris products. Your review will help other researchers decide on the best products for their research. Why not submit a review today?!
Submit ReviewEIPA
Description: Inhibits TRPP3-mediated currents; also inhibits the Na+/H+ exchanger (NHE)
Alternative Names: Ethylisopropyl amiloride
Chemical Name: 3-Amino-N-(aminoiminomethyl)-6-chloro-5-[ethyl(1-methylethyl)amino]-2-pyrazinecarboxamide
Purity: ≥98% (HPLC)
Biological Activity for EIPA
EIPA is a TRPP3 channel inhibitor (IC50 = 10.5 μM). Inhibits the Na+/H+ exchanger (NHE). Derivative of amiloride (Cat. No. 0890).
Compound Libraries for EIPA
EIPA is also offered as part of the Tocriscreen 2.0 Max. Find out more about compound libraries available from Tocris.
Technical Data for EIPA
| M. Wt | 299.76 |
| Formula | C11H18ClN7O |
| Storage | Store at +4°C |
| Purity | ≥98% (HPLC) |
| CAS Number | 1154-25-2 |
| PubChem ID | 1795 |
| InChI Key | QDERNBXNXJCIQK-UHFFFAOYSA-N |
| Smiles | ClC1=C(N(C(C)C)CC)N=C(N)C(C(NC(N)=N)=O)=N1 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for EIPA
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 29.98 | 100 |
Preparing Stock Solutions for EIPA
The following data is based on the product molecular weight 299.76. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 3.34 mL | 16.68 mL | 33.36 mL |
| 5 mM | 0.67 mL | 3.34 mL | 6.67 mL |
| 10 mM | 0.33 mL | 1.67 mL | 3.34 mL |
| 50 mM | 0.07 mL | 0.33 mL | 0.67 mL |
Molarity Calculator
Molarity Calculator
Calculate the mass, volume, or concentration required for a solution.
Reconstitution Calculator
Reconstitution Calculator
Dilution Calculator
Dilution Calculator
Calculate the dilution required to prepare a stock solution.
Product Datasheets for EIPA
References for EIPA
References are publications that support the biological activity of the product.
Dai et al (2007) Inhibition of TRPP3 channel by amil. and analogs. Mol.Pharmacol. 72 1576 PMID: 17804601
Horinouchi et al (2008) Functional role of Na+/H+ exchanger in Ca2+ influx mediated via human endothelin type A receptor stably expressed in Chinese hamster ovary cells. J.Pharm.Sci. 107 456 PMID: 18678984
If you know of a relevant reference for EIPA, please let us know.
View Related Products by Target
View Related Products by Product Action
Keywords: EIPA, EIPA supplier, sodium, hydrogen, exchangers, NHE-1, NHE, blockers, inhibitors, inhibits, TRPP3, antagonists, Amiloride, ethylisopropyl, amiloride, Ethylisopropyl, Na+/H+, Exchanger, TRPP, 3378, Tocris Bioscience
2 Citations for EIPA
Citations are publications that use Tocris products. Selected citations for EIPA include:
Knudsen et al (2019) Dysregulation of Glucagon Secretion by Hyperglycemia-Induced Sodium-Dependent Reduction of ATP Production. Cell Metab 29 430 PMID: 30415925
Neubert et al (2020) NCX1 Represents an Ionic Na+ Sensing Mechanism in Macrophages. PloS Biol 18 PMID: 32569301
Do you know of a great paper that uses EIPA from Tocris? Please let us know.
Reviews for EIPA
Average Rating: 5 (Based on 1 Review.)
Have you used EIPA?
Submit a review and receive an Amazon gift card.
$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Filter by:
EIPA (Cat. No. 3378).
By
Clara Lubeseder-Martellato on 11/12/2021
Assay Type: In Vitro
Species: Mouse
Cell Line/Tissue: prmouse pancreatic tumor cell lines
EIPA was added to the cells at 25 uM for 48 hours