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Description: Fluorescent TG2/LRP1 substrate for imaging endocytosis
Purity: ≥90% (HPLC)
Biological Activity for HB-2-30
Key information: HB-2-30 is a far-red fluorescent transglutaminase-2 (TG2) probe for imaging endocytosis in vitro and in vivo. HB-2-30 undergoes highly efficient endocytosis in complex with TG2 and α2-macroglobulin via the LRP1 pathway.
Application: Confocal microscopy.
Properties and Photophysical Data: Suitable for multiplexing and can be combined with DAPI (Cat. No. 5748) counter-staining. Excitation and emission maxima (λ) are 649 and 665 nm, respectively.
Scientific Data
Confocal Microscopy
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Application of HB-2-30 in vitro. Confocal microscopy images of bone marrow-derived dendritic cells from WT C57BL/6 mice (left) and TG2KO mice (right) incubated with 3μM HB-2-30. Cells were incubated for 90 minutes with the probe before being fixed, stained for nuclei (blue), and imaged. HB-2-30 signals is in red.
Confocal Microscopy
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Application of HB-2-30 in vivo. Ileum tissue sections from mice dosed with 10 mg/kg HB-2-30. WT C57BL/6 mice (left) and TG2KO mice (right) were dosed with HB-2-30 and sacrificed after 2 hours. Intestines were Swiss rolled, thin sectioned, stained for nuclei (blue), and imaged via confocal fluorescence microscopy. HB-2-30 signal is in red.
Licensing Information
Sold under license from Stanford University
Optical Data for HB-2-30
| λabs | 649 nm |
|---|---|
| λem | 665 nm |
| Application | Confocal microscopy |
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectral Viewer
Technical Data for HB-2-30
| M. Wt | 1307.59 |
| Formula | C66H86N10O14S2 |
| Sequence | PXXPF (Modifications: Pro-1 = N-terminal Ac, X-2 = (S,E)-2-amino-7-(dimethylamino)-7-oxohept-5-enoic acid, X-3 = Dab(Sulfo-Cy5), Phe-5 = C-terminal amide) |
| Storage | Store at -20°C |
| Purity | ≥90% (HPLC) |
| CAS Number | 3031522-14-9 |
| InChI Key | ILCHGSXJNAIGGG-AFAVFPMXSA-N |
| Smiles | O=C(N1[C@@H](CCC1)C(N[C@H](C(N)=O)CC2=CC=CC=C2)=O)[C@@H](NC([C@H](CC/C=C/C(N(C)C)=O)NC([C@H]3N(CCC3)C(C)=O)=O)=O)CCNC(CCCCC[N+]4=C(/C=C/C=C/C=C5C(C)(C6=CC(S(=O)(O)=O)=CC=C6N\5C)C)C(C)(C)C7=CC(S(=O)([O-])=O)=CC=C47)=O |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for HB-2-30
| Solubility | Soluble to 13.08 mg/ml in DMSO |
Product Datasheets for HB-2-30
References for HB-2-30
References are publications that support the biological activity of the product.
Loppinet et al (2023) LRP-1 links post-translational modifications to efficient presentation of celiac disease-specific T cell antigens. Cell Chem.Biol. 30 PMID: 36608691
Loppinet et al (2023) Targeted lysosomal degradation of secreted and cell surface proteins through the LRP-1 pathway. J.Am.Chem.Soc. 145 18705 PMID: 37590164
If you know of a relevant reference for HB-2-30, please let us know.
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Keywords: HB-2-30, HB-2-30 supplier, Hb230, fluorescent, probe, probes, TG2, LRP1, α2-macroglobulin, endocytosis, LYTAC, lysosome, targeting, chimera, lysosomal, in, vivo, Fluorescent, Lysosome, Probes, 8831, Tocris Bioscience
Citations for HB-2-30
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