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Description: GFP fluorophore mimic for imaging RNA in live cells; activated by binding to Pepper aptamers; suitable for confocal and two-photon microscopy
Chemical Name: 4-Cyano-α-[[4-[(2-hydroxyethyl)methylamino]phenyl]methylene]benzeneacetonitrile
Purity: ≥98% (HPLC)
Biological Activity for HBC 530
HBC 530 is a mimic of green fluorescent protein (GFP) fluorophore for imaging RNA in live cells. Fluoresces when bound to Pepper aptamer; displays high affinity binding (Kd = 3.5 nM). Suitable for confocal and two-photon microscopy. Confocal excitation maximum = 488 nm, emission maximum = 495 - 595 nm. Two-photon excitation maximum = 976 nm, emission maximum = 475 - 575 nm.
To make a 1000x stock solution, add 200 μL DMSO (Cat. No. 3176).
Optical Data for HBC 530
| λabs | 485 nm |
|---|---|
| λem | 530 nm |
| Extinction Coefficient (ε) | 65300 M-1cm-1 |
| Quantum Yield (φ) | 0.66 |
| Closest Laser line | 488 nm |
| Application | Fluorogen, used with RNA Light-up Aptamer, Confocal Microscopy |
| Alternative For | Cat. No. 6434 |
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Technical Data for HBC 530
| M. Wt | 303.37 |
| Formula | C19H17N3O |
| Storage | Store at -20°C |
| Purity | ≥98% (HPLC) |
| CAS Number | 156840-13-0 |
| InChI Key | DIAVZHWDYXQAFC-LDADJPATSA-N |
| Smiles | CN(CCO)C1=CC=C(C=C1)/C=C(C#N)/C2=CC=C(C=C2)C#N |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for HBC 530
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 30.34 | 100 |
Preparing Stock Solutions for HBC 530
The following data is based on the product molecular weight 303.37. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 3.3 mL | 16.48 mL | 32.96 mL |
| 5 mM | 0.66 mL | 3.3 mL | 6.59 mL |
| 10 mM | 0.33 mL | 1.65 mL | 3.3 mL |
| 50 mM | 0.07 mL | 0.33 mL | 0.66 mL |
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Product Datasheets for HBC 530
References for HBC 530
References are publications that support the biological activity of the product.
Chen et al (2019) Visualizing RNA dynamics in live cells with bright and stable fluorescent RNAs. Nat.Biotechnol. 37 1287 PMID: 31548726
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Citations for HBC 530
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Fluorescent Dyes and Probes Research Product Guide
This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:
- Fluorescent Dyes, including dyes for flow cytometry
- Fluorescent Probes and Stains, including our new MitoBrilliantTM mitochondria stains
- Tissue Clearing Kits and Reagents
- Aptamer-based RNA Imaging Reagents
- Fluorescent Probes for Imaging Bacteria
- TSA VividTM Fluorophore Kits
- TSA Reagents for Enhancing IHC, ICC & FISH Signals