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Submit ReviewHygromycin B
Description: Aminoglycoside antibiotic; active against prokaryotic and eukaryotic cells
Chemical Name: O-6-Amino-6-deoxy-L-glycero-D-galacto-heptopyranosylidene-(1-2-3)-O-β-D-talopyranosyl(1-5)-2-deoxy-N3-methyl-D-streptamine
Purity: ≥90% (HPLC)
Biological Activity for Hygromycin B
Hygromycin B is an aminoglycoside antibiotic. Active against both prokaryotic and eukaryotic cells. Inhibits protein synthesis by binding to the 30S subunit and inhibiting the ribosomal translocation step of elongation.
Technical Data for Hygromycin B
| M. Wt | 527.52 |
| Formula | C20H37N3O13 |
| Storage | Store at +4°C |
| Purity | ≥90% (HPLC) |
| CAS Number | 31282-04-9 |
| PubChem ID | 90488914 |
| InChI Key | GRRNUXAQVGOGFE-SZNRJEGWSA-N |
| Smiles | O[C@H]([C@@H](NC)C[C@@H](N)[C@@H]4O)C4O[C@H]1[C@]2([H])[C@](OC3(O[C@]([H])([C@@H](N)CO)[C@H](O)[C@@H](O)[C@H]3O)O2)([H])[C@@H](O)[C@@H](CO)O1 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for Hygromycin B
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| water | 50 | 95 |
Preparing Stock Solutions for Hygromycin B
The following data is based on the product molecular weight 527.52. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.95 mM | 2 mL | 9.98 mL | 19.95 mL |
| 4.75 mM | 0.4 mL | 2 mL | 3.99 mL |
| 9.5 mM | 0.2 mL | 1 mL | 2 mL |
| 47.5 mM | 0.04 mL | 0.2 mL | 0.4 mL |
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Product Datasheets for Hygromycin B
References for Hygromycin B
References are publications that support the biological activity of the product.
Blochlinger and Diggelmann (1984) Hygromycin B phosphotransferase as a selectable marker for DNA transfer experiments with higher eukaryotic cells. Mol.Cell.Biol. 4 2929 PMID: 6098829
Brodersen et al (2000) The structural basis for the action of the antibiotics tetracycline, pactamycin, and hygromycin B on the 30S ribosomal subunit. Cell 103 1143 PMID: 11163189
Ganoza and Kiel (2001) A ribosomal ATPase is a target for hygromycin B inhibition on Escherichia coli ribosomes. Antimicrob.Agents Chemother. 45 2813 PMID: 11557474
If you know of a relevant reference for Hygromycin B, please let us know.
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Keywords: Hygromycin B, Hygromycin B supplier, hygromycinB, aminoglycoside, antibiotics, inhibits, protein, synthesis, ribosomal, translocation, ribosome, 30S, eukaryotic, prokaryotic, cells, Antibiotics, 4137, Tocris Bioscience
Citations for Hygromycin B
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Reviews for Hygromycin B
Average Rating: 5 (Based on 1 Review.)
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Hygromycin used as a selection marker in cells engineered with Hygromycin resistant gene.
By
Anonymous on 11/15/2018
Assay Type: In Vitro
Species: Human
Hygromycin was used as a selection marker for few eukaryotic cells which were engineered with Hygromycin resistant gene. Product works beautifully to inhibit translation and killing non resistant strains giving it desirable specificity.
