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Description: Macrophage migration inhibitory factor (MIF) inhibitor
Chemical Name: 4,5-Dihydro-3-(4-hydroxyphenyl)-5-isoxazoleacetic acid methyl ester
Purity: ≥98% (HPLC)
Biological Activity for ISO 1
ISO 1 is a macrophage migration inhibitory factor (MIF) inhibitor (IC50 = 7 μM); inhibits MIF tautomerase activity in vitro and in vivo. Protective against mouse models of streptozotocin-induced diabetes mellitus; blocks anti-inflammatory response following LPS exposure and increases survival. Inhibits airway remodeling in a mouse model of chronic asthma. Cell permeable; orally bioavailable.
Compound Libraries for ISO 1
ISO 1 is also offered as part of the Tocriscreen 2.0 Max and Tocriscreen Antiviral Library. Find out more about compound libraries available from Tocris.
Technical Data for ISO 1
| M. Wt | 235.24 |
| Formula | C12H13NO4 |
| Storage | Store at +4°C |
| Purity | ≥98% (HPLC) |
| CAS Number | 478336-92-4 |
| PubChem ID | 6098948 |
| InChI Key | CLVQGFPQFNASJH-UHFFFAOYSA-N |
| Smiles | OC(C=C2)=CC=C2C1=NOC(CC(OC)=O)C1 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for ISO 1
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 23.52 | 100 | |
| ethanol | 23.52 | 100 |
Preparing Stock Solutions for ISO 1
The following data is based on the product molecular weight 235.24. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 4.25 mL | 21.25 mL | 42.51 mL |
| 5 mM | 0.85 mL | 4.25 mL | 8.5 mL |
| 10 mM | 0.43 mL | 2.13 mL | 4.25 mL |
| 50 mM | 0.09 mL | 0.43 mL | 0.85 mL |
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Product Datasheets for ISO 1
References for ISO 1
References are publications that support the biological activity of the product.
Al-Abed et al (2005) ISO-1 binding to the tautomerase active site of MIF inhibits its pro-inflammatory activity and increases survival in severe sepsis. J.Biol.Chem. 280 36541 PMID: 16115897
Cvetkovic et al (2005) Critical role of macrophage migration inhibitory factor activity in experimental autoimmune diabetes. Endocrinology 146 2942 PMID: 15790730
Chen et al (2010) ISO-1, a macrophage migration inhibitory factor antagonist, inhibits airway remodeling in a murine model of chronic asthma. Mol.Med. 16 400 PMID: 20485865
Lubetsky et al (2002) The tautomerase active site of macrophage migration inhibitory factor is a potential target for discovery of novel anti-inflammatory agents. J.Biol.Chem. 277 24976 PMID: 11997397
If you know of a relevant reference for ISO 1, please let us know.
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Keywords: ISO 1, ISO 1 supplier, ISO1, macrophage, migration, inhibitory, factor, antagonists, inhibits, inhibitors, tautomerase, MIF, Cytokines, 4288, Tocris Bioscience
4 Citations for ISO 1
Citations are publications that use Tocris products. Selected citations for ISO 1 include:
Heide L et al (2022) Sine oculis homeobox homolog 1 plays a critical role in pulmonary fibrosis. JCI Insight 7 PMID: 35420997
Graham et al (2015) The expression of microRNA-451 in human endometriotic lesions is inversely related to that of macrophage migration inhibitory factor (MIF) and regulates MIF expression and modulation of epithelial cell survival. Hum Reprod 30 642 PMID: 25637622
Julio et al (2020) Autophagy Inhibition Induces the Secretion of Macrophage Migration Inhibitory Factor (MIF) with Autocrine and Paracrine Effects on the Promotion of Malignancy in Breast Cancer. Biology (Basel) 9 PMID: 31963754
Justin D et al (2020) Glioblastoma Myeloid-Derived Suppressor Cell Subsets Express Differential Macrophage Migration Inhibitory Factor Receptor Profiles That Can Be Targeted to Reduce Immune Suppression. Front Immunol 11 1191 PMID: 32625208
Do you know of a great paper that uses ISO 1 from Tocris? Please let us know.
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