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Submit ReviewJanelia Fluor® 525, Haloalkane New
Description: Cell-permeable fluorogenic fluorescent dye with a chloroalkane handle. Application: live-cell imaging as a self-labeling tag substrate
Chemical Name: 4-((2-(2-((6-Chlorohexyl)oxy)ethoxy)ethyl)carbamoyl)-2-(3-(3,3-difluoroazetidin-1-ium-1-ylidene)-6-(3,3-difluoroazetidin-1-yl)-3H-xanthen-9-yl)benzoate
Purity: ≥95% (HPLC)
Biological Activity for Janelia Fluor® 525, Haloalkane
Key Information: Janelia Fluor® 525, Haloalkane is a yellow cell-permeable fluorogenic fluorescent dye with a chloroalkane handle.
Application: Live-cell imaging as a self-labeling tag substrate. Suitable for confocal microscopy, light sheet microscropy, super resolution microscopy (SRM) techniques including dSTORM (in both live and fixed cells). Stable to fixation. Cell permeable.
Properties and Photophysical Data: Janelia Fluor® 525, Haloalkane shows a high degree of fluorogenicity. Excitation and emission maxima (λ) are 525 nm and 549 nm, respectively; quantum yield = 0.91; extinction coefficient = 122,000 M-1cm-1 (measured in ethanol or TFE plus 0.1% TFA); lactone-zwitterion equilibrium constant (KL-Z) = 0.068. Exhibits low non-specific background staining.
Janelia Fluor is a registered trademark of Howard Hughes Medical Institute.
Licensing Information
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus
Optical Data for Janelia Fluor® 525, Haloalkane
| Emission Color | Yellow |
|---|---|
| λabs | 525 nm |
| λem | 549 nm |
| Extinction Coefficient (ε) | 122,000 M-1cm-1 |
| Quantum Yield (φ) | 0.91 |
| Closest Laser line | 488 nm |
| Cell Permeable | Yes |
| Application | Confocal Microscopy, Super Resolution Microscopy (SRM) |
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Technical Data for Janelia Fluor® 525, Haloalkane
| M. Wt | 732.17 |
| Formula | C37H38ClF4N3O6 |
| Storage | Store at -20°C |
| Purity | ≥95% (HPLC) |
| CAS Number | 1811539-37-3 |
| PubChem ID | 140772613 |
| InChI Key | TZCOVYGWYYWODF-UHFFFAOYSA-N |
| Smiles | O=C(C1=C(C(C2=C(OC3=C/4)C=C(N5CC(F)(C5)F)C=C2)=C3C=CC4=[N+]6CC(F)(C\6)F)C=C(C(NCCOCCOCCCCCCCl)=O)C=C1)[O-] |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for Janelia Fluor® 525, Haloalkane
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 7.32 | 10 |
Preparing Stock Solutions for Janelia Fluor® 525, Haloalkane
The following data is based on the product molecular weight 732.17. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.1 mM | 13.66 mL | 68.29 mL | 136.58 mL |
| 0.5 mM | 2.73 mL | 13.66 mL | 27.32 mL |
| 1 mM | 1.37 mL | 6.83 mL | 13.66 mL |
| 5 mM | 0.27 mL | 1.37 mL | 2.73 mL |
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Product Datasheets for Janelia Fluor® 525, Haloalkane
References for Janelia Fluor® 525, Haloalkane
References are publications that support the biological activity of the product.
Grimm et al (2017) A general method to fine-tune fluorophores for live-cell and in vivo imaging. Nat. Methods 17 987 PMID: 28869757
If you know of a relevant reference for Janelia Fluor® 525, Haloalkane, please let us know.
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