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JB 300

Cat. No. 7837 Submit a Review Datasheet / COA / SDS
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Description: Aurora A Degrader (PROTAC®)
Chemical Name: trans-4-(3-Chloro-2-fluorophenoxy)-N-(2-(2-(2-(2-((2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindolin-4-yl)oxy)acetamido)ethoxy)ethoxy)ethyl)-1-((6-(thiazol-2-ylamino)pyridin-2-yl)methyl)cyclohexane-1-carboxamide
Purity: ≥98% (HPLC)
Datasheet
Citations
Reviews
Literature (2)
Biological Activity Technical Data Solubility Calculators Datasheets References

Biological Activity for JB 300

JB 300 is a potent and selective Aurora A Degrader (PROTAC®; DC50 = 30 nM and Dmax = 78%). It comprises a cereblon E3-ligase ligand Thalidomide (Cat. No. 0652) joined by a PEG2 linker to the Aurora A inhibitor MK-5108. The compound depletes Aurora A in MV4-11 cell lines and exhibits no degradation of Aurora B at 10 µM. JB 300 shows dose-dependent cytotoxicity in AML cells.

Aurora A antibodies validated for Simple Western™ (automated Western) instruments and Western Blot also available: Catalog # AF3295 and NBP1-51843.

PROTAC® is a registered trademark of Arvinas Operations, Inc., and is used under license.

Licensing Information

Sold under license from University of Würzburg

Technical Data for JB 300

M. Wt 906.38
Formula C43H45ClFN7O10S
Storage Store at -20°C
Purity ≥98% (HPLC)
CAS Number 3038446-90-8
InChI Key LAZHTWDJEQSMOY-NKEKBGIASA-N
Smiles O=C([C@@]1(CC2=CC=CC(NC3=NC=CS3)=N2)CC[C@H](OC4=C(F)C(Cl)=CC=C4)CC1)NCCOCCOCCNC(COC5=C6C(N(C7CCC(NC7=O)=O)C(C6=CC=C5)=O)=O)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for JB 300

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 18.13 20

Preparing Stock Solutions for JB 300

The following data is based on the product molecular weight 906.38. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.2 mM 5.52 mL 27.58 mL 55.16 mL
1 mM 1.1 mL 5.52 mL 11.03 mL
2 mM 0.55 mL 2.76 mL 5.52 mL
10 mM 0.11 mL 0.55 mL 1.1 mL

Molarity Calculator

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Reconstitution Calculator

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References for JB 300

References are publications that support the biological activity of the product.

Adhikari et al (2020) PROTAC-mediated degradation reveals a non-catalytic function of AURORA-A kinase. Nat.Chem.Biol. 16 1179 PMID: 32989298

Bozolovic et al (2022) Novel, highly potent PROTACs targeting AURORA-A kinase. Curr.Res.Chem.Biol. 2 100032

If you know of a relevant reference for JB 300, please let us know.

View Related Products by Target

Keywords: JB 300, JB 300 supplier, JB300, AURORA-A, PROTACs, Degraders, degrades, kinase., Aurora, Kinases, Kinase, 7837, Tocris Bioscience

Citations for JB 300

Citations are publications that use Tocris products.

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Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

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TPD and Induced Proximity Research Product Guide

TPD and Induced Proximity Research Product Guide

This brochure highlights the tools and services available from Bio-Techne to support your Targeted Protein Degradation and Induced Proximity research, including:

  • Active Degraders
  • TAG Degradation Platform
  • Degrader Building Blocks
  • Assays for Protein Degradation
  • Induced Proximity Tools
Targeted Protein Degradation Poster

Targeted Protein Degradation Poster

Degraders (e.g. PROTACs) are bifunctional small molecules, that harness the Ubiquitin Proteasome System (UPS) to selectively degrade target proteins within cells. They consist of three covalently linked components: an E3 ubiquitin ligase ligand, a linker and a ligand for the target protein of interest. Authored in-house, this poster outlines the generation of a toolbox of building blocks for the development of Degraders. The characteristics and selection of each of these components are discussed. Presented at EFMC 2018, Ljubljana, Slovenia