Submit a Review & Earn an Amazon Gift Card
You can now submit reviews for your favorite Tocris products. Your review will help other researchers decide on the best products for their research. Why not submit a review today?!
Submit ReviewJW 74
Description: Wnt pathway inhibitor
Chemical Name: 4-[4-(4-Methoxyphenyl)-5-[[[3-(4-methylphenyl)-1,2,4-oxadiazol-5-yl]methyl]thio]-4H-1,2,4-triazol-3-yl]-pyridine
Purity: ≥99% (HPLC)
Biological Activity for JW 74
JW 74 is an inhibitor of canonical Wnt signaling (IC50 = 790 nM). Targets the β-catenin destruction complex (GSK-3 beta /AXIN/APC). Increases AXIN2 and decreases active β-catenin levels in colorectal cancer (CRC) cells. Also arrests cell cycle in G1 and inhibits proliferation of CRC cells in vitro. Inhibits tumor growth and formation in mouse models.
Compound Libraries for JW 74
JW 74 is also offered as part of the Tocriscreen 2.0 Max and Tocriscreen Stem Cell Library. Find out more about compound libraries available from Tocris.
Technical Data for JW 74
| M. Wt | 456.52 |
| Formula | C24H20N6O2S |
| Storage | Store at +4°C |
| Purity | ≥99% (HPLC) |
| CAS Number | 863405-60-1 |
| PubChem ID | 16013152 |
| InChI Key | KRIKILRRJCIWNB-UHFFFAOYSA-N |
| Smiles | COC1=CC=C(N2C(SCC3=NC(C4=CC=C(C)C=C4)=NO3)=NN=C2C5=CC=NC=C5)C=C1 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for JW 74
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 45.65 | 100 |
Preparing Stock Solutions for JW 74
The following data is based on the product molecular weight 456.52. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 2.19 mL | 10.95 mL | 21.9 mL |
| 5 mM | 0.44 mL | 2.19 mL | 4.38 mL |
| 10 mM | 0.22 mL | 1.1 mL | 2.19 mL |
| 50 mM | 0.04 mL | 0.22 mL | 0.44 mL |
Molarity Calculator
Molarity Calculator
Calculate the mass, volume, or concentration required for a solution.
Reconstitution Calculator
Reconstitution Calculator
Dilution Calculator
Dilution Calculator
Calculate the dilution required to prepare a stock solution.
Product Datasheets for JW 74
References for JW 74
References are publications that support the biological activity of the product.
Waaler et al (2011) Novel synthetic antagonists of canonical Wnt signaling inhibit colorectal cancer cell growth. Cancer Res. 71 197 PMID: 21199802
If you know of a relevant reference for JW 74, please let us know.
View Related Products by Target
View Related Products by Product Action
Keywords: JW 74, JW 74 supplier, JW74, canonical, Wnt, signaling, pathway, inhibitors, inhibits, cancer, β-catenin, AXIN2, Beta-catenin, Other, Signaling, 5653, Tocris Bioscience
1 Citation for JW 74
Citations are publications that use Tocris products. Selected citations for JW 74 include:
Rothberg (2018) Mtf2-PRC2 control of canonical Wnt signaling is required for definitive erythropoiesis. Cell Discov 4 21 PMID: 29736258
Do you know of a great paper that uses JW 74 from Tocris? Please let us know.
Reviews for JW 74
There are currently no reviews for this product. Be the first to review JW 74 and earn rewards!
Have you used JW 74?
Submit a review and receive an Amazon gift card.
$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Pathways for JW 74
