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ML 67-33

Cat. No. 6886 1 Citation Submit a Review Datasheet / COA / SDS
Pricing Availability   Qty
Description: K2P2.1 (TREK-1), K2P10.1 (TREK-2) and K2P4.1 (TRAAK) channel activator
Chemical Name: 2,7-Dichloro-9,10-dihydro-9,9-dimethyl-10-[2-(2H-tetrazol-5-yl)ethyl]acridine
Purity: ≥98% (HPLC)
Datasheet
Citations (1)
Reviews
Biological Activity Technical Data Solubility Calculators Datasheets References

Biological Activity for ML 67-33

ML 67-33 is a K2P potassium channel activator (EC50 values are 21.8 - 29.4 μM, 30.2 μM and 27.3 μM for K2P2.1 (TREK-1), K2P10.1 (TREK-2) and K2P4.1 (TRAAK) respectively, expressed in xenopus oocytes). Increases channel currents by activating core gating apparatus of channels. In a mouse migraine model, ML 67-33 improves pain symptoms via activation of TREK 1/2 currents in trigeminal ganglion sensory neurons.

Compound Libraries for ML 67-33

ML 67-33 is also offered as part of the Tocriscreen 2.0 Max. Find out more about compound libraries available from Tocris.

Technical Data for ML 67-33

M. Wt 374.27
Formula C18H17Cl2N5
Storage Store at -20°C
Purity ≥98% (HPLC)
CAS Number 1443290-89-8
PubChem ID 86272454
InChI Key GQPMJOXQJUGJPG-UHFFFAOYSA-N
Smiles CC1(C2=CC(Cl)=CC=C2N(C3=CC=C(C=C13)Cl)CCC4=NN=NN4)C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for ML 67-33

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 37.43 100

Preparing Stock Solutions for ML 67-33

The following data is based on the product molecular weight 374.27. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.67 mL 13.36 mL 26.72 mL
5 mM 0.53 mL 2.67 mL 5.34 mL
10 mM 0.27 mL 1.34 mL 2.67 mL
50 mM 0.05 mL 0.27 mL 0.53 mL

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Product Datasheets for ML 67-33

Certificate of Analysis / Product Datasheet
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Safety Datasheet

References for ML 67-33

References are publications that support the biological activity of the product.

Bagriantsev et al (2013) A high-throughput functional screen identifies small molecule regulators of temperature- and mechano-sensitive K2P channels. ACS Chem.Biol. 8 1841 PMID: 23738709

Pope et al (2018) Protein and chemical determinants of BL-1249 action and selectivity for K2P channels. ACS Chem.Neurosci. 9 3153 PMID: 30089357

Prado et al (2021) TREK channel activation suppresses migraine pain phenotype. iScience 24 102961 PMID: 34458705

If you know of a relevant reference for ML 67-33, please let us know.

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1 Citation for ML 67-33

Citations are publications that use Tocris products. Selected citations for ML 67-33 include:

Prado et al (2021) TREK channel activation suppresses migraine pain phenotype. iScience 24 102961 PMID: 34458705

Do you know of a great paper that uses ML 67-33 from Tocris? Please let us know.

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