Home / Pharmacology / Enzyme-Linked Receptors / Receptor Serine/Threonine Kinases (RSTKs) / TGF-β Receptors / N-Acetylpuromycin

N-Acetylpuromycin

Cat. No. 5679 2 Citations Submit a Review Datasheet / COA / SDS
Pricing Availability   Qty
Description: Downregulates SnoN and Ski protein expression; promotes TGF-β signaling
Chemical Name: 3'-[[(2S)-2-(Acetylamino)-3-(4-methoxyphenyl)-1-oxopropyl]amino]-3'-deoxy-N,N-dimethyladenosine
Purity: ≥98% (HPLC)
Datasheet
Citations (2)
Reviews
Literature (3)
Pathways (1)
Biological Activity Technical Data Solubility Calculators Datasheets References

Biological Activity for N-Acetylpuromycin

N-Acetylpuromycin downregulates SnoN and Ski protein expression; promotes TGF-β signaling, independently of MAPK activation. Does not bind ribosomes or block protein synthesis.

Technical Data for N-Acetylpuromycin

M. Wt 513.55
Formula C24H31N7O6
Storage Store at -20°C
Purity ≥98% (HPLC)
CAS Number 22852-13-7
PubChem ID 46173769
InChI Key LADKVYSQIGJMFP-IYRMOJGWSA-N
Smiles O[C@@H]1[C@H](NC([C@@H](NC(C)=O)CC4=CC=C(OC)C=C4)=O)[C@@H](CO)O[C@H]1N3C2=NC=NC(N(C)C)=C2N=C3

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for N-Acetylpuromycin

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 51.35 100
1eq. HCl 51.35 100

Preparing Stock Solutions for N-Acetylpuromycin

The following data is based on the product molecular weight 513.55. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.95 mL 9.74 mL 19.47 mL
5 mM 0.39 mL 1.95 mL 3.89 mL
10 mM 0.19 mL 0.97 mL 1.95 mL
50 mM 0.04 mL 0.19 mL 0.39 mL

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Product Datasheets for N-Acetylpuromycin

Certificate of Analysis / Product Datasheet
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Safety Datasheet

References for N-Acetylpuromycin

References are publications that support the biological activity of the product.

Hernández-Damián et al (2013) Downregulation of SnoN oncoprotein induced by antibiotics anisomycin and puromycin positively regulates transforming growth factor-β signals. Biochim.Biophys.Acta. 1830 5049 PMID: 23872350

If you know of a relevant reference for N-Acetylpuromycin, please let us know.

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Keywords: N-Acetylpuromycin, N-Acetylpuromycin supplier, SnoN, SKI, proteins, downregulates, downregulation, transforming, growth, factor, beta, TGF-beta, TGF-B, TGF-β, signaling, ribosomes, Receptors, 5679, Tocris Bioscience

2 Citations for N-Acetylpuromycin

Citations are publications that use Tocris products. Selected citations for N-Acetylpuromycin include:

Alun R et al (2020) A Semiautomated, Phenotypic, In Vitro Scratch Assay for Assessing Retinal Pigment Epithelial Cell Wound Healing. J Ocul Pharmacol Ther 36 257-266 PMID: 32027217

Hossein et al (2020) Directed differentiation of regulatory T cells from naive T cells and prevention of their inflammation-mediated instability using small molecules. Clin Exp Immunol 201 205-221 PMID: 32403163

Do you know of a great paper that uses N-Acetylpuromycin from Tocris? Please let us know.

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Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.

Stem Cell Research Product Guide

Stem Cell Research Product Guide

This product guide provides a background to the use of small molecules in stem cell research and lists over 200 products for use in:

  • Self-renewal and Maintenance
  • Differentiation
  • Reprogramming
  • Organoid Generation
  • GMP and Ancillary Material Grade Products
Stem Cells Scientific Review

Stem Cells Scientific Review

Written by Kirsty E. Clarke, Victoria B. Christie, Andy Whiting and Stefan A. Przyborski, this review provides an overview of the use of small molecules in the control of stem cell growth and differentiation. Key signaling pathways are highlighted, and the regulation of ES cell self-renewal and somatic cell reprogramming is discussed. Compounds available from Tocris are listed.

Stem Cells Poster

Stem Cells Poster

Written by Rebecca Quelch and Stefan Przyborski from Durham University (UK), this poster describes the isolation of pluripotent stem cells, their maintenance in culture, differentiation, and the generation and potential uses of organoids.

Pathways for N-Acetylpuromycin

TGF-β Signaling Pathway

TGF-β Signaling Pathway

The TGF-β signaling pathway is involved in the regulation of growth and proliferation of cells along with migration, differentiation and apoptosis.