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Submit ReviewNF 546
Description: Selective P2Y11 agonist
Chemical Name: 4,4'-(Carbonylbis(imino-3,1-phenylene-carbonylimino-3,1-(4-methyl-phenylene)carbonylimino))-bis(1,3-xylene-alpha,alpha'-diphosphonic acid tetrasodium salt
Purity: ≥95% (HPLC)
Biological Activity for NF 546
NF 546 is a P2Y11 agonist (pEC50 = 6.27). Exhibits selectivity for P2Y11 over a range of P2Y and P2X receptors. Also inhibits DNA-binding activity of HMGA2 (IC50 = 5.49 μM).
Technical Data for NF 546
| M. Wt | 1180.74 |
| Formula | C47H44N6Na4O17P4 |
| Storage | Store at -20°C |
| Purity | ≥95% (HPLC) |
| CAS Number | 1006028-37-0 |
| PubChem ID | 73755075 |
| InChI Key | GSMUPMANKNAMAS-UHFFFAOYSA-J |
| Smiles | [Na+].[Na+].[Na+].[Na+].CC1=CC=C(C=C1NC(=O)C1=CC=CC(NC(=O)NC2=CC=CC(=C2)C(=O)NC2=C(C)C=CC(=C2)C(=O)NC2=CC=C(CP(O)([O-])=O)C=C2CP(O)([O-])=O)=C1)C(=O)NC1=C(CP(O)([O-])=O)C=C(CP(O)([O-])=O)C=C1 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for NF 546
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| water | 11.81 | 10 with gentle warming |
Preparing Stock Solutions for NF 546
The following data is based on the product molecular weight 1180.74. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.1 mM | 8.47 mL | 42.35 mL | 84.69 mL |
| 0.5 mM | 1.69 mL | 8.47 mL | 16.94 mL |
| 1 mM | 0.85 mL | 4.23 mL | 8.47 mL |
| 5 mM | 0.17 mL | 0.85 mL | 1.69 mL |
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Product Datasheets for NF 546
References for NF 546
References are publications that support the biological activity of the product.
Meis et al (2010) NF546 [4,4'-(carbonylbis(imino-3,1-phenylene-carbonylimino-3,1-(4-methyl-phenylene)-carbonylimino))-bis(1,3-xylene-γ,γ'-diphosphonic acid) tetrasodium salt] is a non-nucleotide P2Y11 agonist and stimulates release of interleuk J.Pharmacol.Exp.Ther. 332 238 PMID: 19815812
Su et al (2020) Identification of HMGA2 inhibitors by AlphaScreen-based ultra-high-throughput screening assays. Sci.Rep. 10 18850 PMID: 33139812
If you know of a relevant reference for NF 546, please let us know.
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Keywords: NF 546, NF 546 supplier, NF546, p2y11, purinergic, receptors, agonists, agonism, HMGA2, inhibitors, inhibits, high, mobility, group, protein, AT-hook, 2, DNA, binding, Purinergic, P2Y, Receptors, 3892, Tocris Bioscience
5 Citations for NF 546
Citations are publications that use Tocris products. Selected citations for NF 546 include:
Barragán-Iglesias et al (2014) Role of spinal P2Y6 and P2Y11 receptors in neuropathic pain in rats: possible involvement of glial cells. Mol Pain 10 29 PMID: 24886406
Gao et al (2013) The role of P2Y(14) and other P2Y receptors in degranulation of human LAD2 mast cells. Sci Adv 9 31 PMID: 22825617
Johannes W et al (2019) A Gs-coupled purinergic receptor boosts Ca2+ influx and vascular contractility during diabetic hyperglycemia. Elife 8 PMID: 30821687
Su et al (2020) Identification of HMGA2 inhibitors by AlphaScreen-based ultra-high-throughput screening assays. Sci Rep 10 18850 PMID: 33139812
Timothy S et al (2021) Differential Regulation of ATP- and UTP-Evoked Prostaglandin E2 and IL-6 Production from Human Airway Epithelial Cells. J Immunol 207 1275-1287 PMID: 34389624
Do you know of a great paper that uses NF 546 from Tocris? Please let us know.
Reviews for NF 546
Average Rating: 4 (Based on 1 Review.)
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Cell growth regulator.
By
Anonymous on 06/10/2019
Assay Type: In Vitro
Species: Human
Cell Line/Tissue: H69
Used this compound in various concentrations (5-30 uM) to check the cell growth in H69 cells by InCucyte.
Literature in this Area
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P2X and P2Y Receptors Scientific Review
Written by Kenneth Jacobson, this review provides an overview of the different subtypes and structures of the P2 receptor families, as well as the pharmacological probes used to study them; compounds available from Tocris are listed.