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Submit ReviewNNC 55-0396 dihydrochloride
Description: Highly selective CaV3.x blocker
Chemical Name: (1S,2S)-2-[2-[[3-(1H-Benzimidazol-2-yl)propyl]methylamino]ethyl]-6-fluoro-1,2,3,4-tetrahydro-1-(1-methylethyl)-2-naphthalenyl cyclopropanecarboxylate dihydrochloride
Purity: ≥98% (HPLC)
Biological Activity for NNC 55-0396 dihydrochloride
NNC 55-0396 dihydrochloride is a highly selective T-type calcium channel blocker. Displays IC50 values of 6.8 and > 100 μM for inhibition of Cav3.1 T-type channels and HVA currents respectively in INS-1 cells.
Technical Data for NNC 55-0396 dihydrochloride
| M. Wt | 564.57 |
| Formula | C30H38FN3O2.2HCl |
| Storage | Desiccate at RT |
| Purity | ≥98% (HPLC) |
| CAS Number | 357400-13-6 |
| PubChem ID | 22084904 |
| InChI Key | BCCQNBXHUMKLFW-HNQRYHMESA-N |
| Smiles | FC1=CC(CC[C@@](CCN(C)CCCC3=NC(C=CC=C5)=C5N3)(OC(C4CC4)=O)[C@H]2[C@H](C)C)=C2C=C1.Cl.Cl |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for NNC 55-0396 dihydrochloride
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| water | 14.11 | 25 |
Preparing Stock Solutions for NNC 55-0396 dihydrochloride
The following data is based on the product molecular weight 564.57. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.25 mM | 7.09 mL | 35.43 mL | 70.85 mL |
| 1.25 mM | 1.42 mL | 7.09 mL | 14.17 mL |
| 2.5 mM | 0.71 mL | 3.54 mL | 7.09 mL |
| 12.5 mM | 0.14 mL | 0.71 mL | 1.42 mL |
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Product Datasheets for NNC 55-0396 dihydrochloride
References for NNC 55-0396 dihydrochloride
References are publications that support the biological activity of the product.
Huang et al (2004) NNC 55-0396 [(1S,2S)-2-(2-(N-[(3-benzimidazol-2-yl)propyl]-N-methylamino)ethyl)-6-fluoro-1,2,3,4-tetrahydro-1-isopropyl-2-naphtyl cyclopropanecarboxylate dihydrochloride]: A new selective inhibitor of T-type calcium cha J.Pharmacol.Exp.Ther. 309 193 PMID: 14718587
Chen et al (2010) CaV3.2 T-type Ca2+ channel-dependent activation of ERK in paraventricular thalamus modulates acid-induced chronic muscle pain. J.Neurosci. 30 10360 PMID: 20685979
If you know of a relevant reference for NNC 55-0396 dihydrochloride, please let us know.
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Keywords: NNC 55-0396 dihydrochloride, NNC 55-0396 dihydrochloride supplier, selective, Ca2+, channel, blockers, T-type, Calcium, CaV, Channels, T-Type, voltage-gated, voltage-dependent, NNC550396, dihydrochloride, Cav3.x, 2268, Tocris Bioscience
9 Citations for NNC 55-0396 dihydrochloride
Citations are publications that use Tocris products. Selected citations for NNC 55-0396 dihydrochloride include:
Senadheera et al (2013) Enhanced contractility in pregnancy is associated with augmented TRPC3, L-type, and T-type voltage-dependent calcium channel function in rat uterine radial artery. Proc Natl Acad Sci U S A 305 R917 PMID: 23948776
Goswami et al (2012) Miniature IPSCs in hippocampal granule cells are triggered by voltage-gated Ca2+ channels via microdomain coupling. J Neurosci 32 14294 PMID: 23055500
Schneider et al (2013) Calcium-based dendritic excitability and its regulation in the deep cerebellar nuclei. J Neurophysiol 109 2282 PMID: 23427305
Chen et al (2010) Ca(v)3.2 T-type Ca2+ channel-dependent activation of ERK in paraventricular thalamus modulates acid-induced chronic muscle pain. J Neurosci 30 10360 PMID: 20685979
Tzeng et al (2012) The Ca(v)3.1 T-type calcium channel is required for neointimal formation in response to vascular injury in mice. Cardiovasc Res 96 533 PMID: 22886848
Kuo et al (2010) Dihydropyridine-insensitive calcium currents contribute to function of small cerebral arteries. J Cereb Blood Flow Metab 30 1226 PMID: 20125181
Baillie et al (2012) Sumatriptan inhibition of N-type calcium channel mediated signaling in dural CGRP terminal fibres. Am J Physiol Regul Integr Comp Physiol 63 362 PMID: 22691374
Mundt et al (2018) TRPV4 is the temperature-sensitive ion channel of human sperm. Elife 7 PMID: 29963982
Abe et al (2011) The Etv1/Er81 transcription factor orchestrates activity-dependent gene regulation in the terminal maturation program of cerebellar granule cells. PLoS One 108 12497 PMID: 21746923
Do you know of a great paper that uses NNC 55-0396 dihydrochloride from Tocris? Please let us know.
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