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PARPYnD

Cat. No. 7410 Submit a Review Datasheet / COA / SDS
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Description: PARP inhibitor; photoaffinity probe
Chemical Name: 3-(3-Butyn-1-yl)-N-[5-cyano-6-[4-[3-[(3,4-dihydro-4-oxo-1-phthalazinyl)methyl]benzoyl]-1-piperazinyl]-3-pyridinyl]-3H-diazirine-3-propanamide
Purity: ≥95% (HPLC)
Datasheet
Citations
Reviews
Literature (2)
Biological Activity Technical Data Solubility Calculators Datasheets References

Biological Activity for PARPYnD

PARPYnD is a potent photoaffinity probe (AfBP) for poly(ADP-ribose) polymerase (PARP) (IC50 values for PARP2, PARP1 and PARP6 are 6, 38 and 230 nM, respectively). Can be used to fluorescently label PARP1 and PARP2 in cells when an azide functionalized probe is attached via copper catalysed click-chemistry. PARPYnD enriches recombinant PARP6 spiked into cellular lysates and inhibits PARP6 in cell-free assays; does not label PARP6 in intact cells. Photocrosslinking can be performed by irradiating at 365 nm.

Technical Data for PARPYnD

M. Wt 613.68
Formula C34H31N9O3
Storage Store at -20°C
Purity ≥95% (HPLC)
CAS Number 2561483-27-8
InChI Key ZYWZRMXOMDKHKX-UHFFFAOYSA-N
Smiles O=C(NC1=CN=C(C(C#N)=C1)N2CCN(C(C3=CC(CC4=NNC(C5=CC=CC=C45)=O)=CC=C3)=O)CC2)CCC6(N=N6)CCC#C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for PARPYnD

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 61.37 100

Preparing Stock Solutions for PARPYnD

The following data is based on the product molecular weight 613.68. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.63 mL 8.15 mL 16.3 mL
5 mM 0.33 mL 1.63 mL 3.26 mL
10 mM 0.16 mL 0.81 mL 1.63 mL
50 mM 0.03 mL 0.16 mL 0.33 mL

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References for PARPYnD

References are publications that support the biological activity of the product.

Howard et al (2020) Structure-guided design and in-cell target profiling of a cell-active target engagement probe for PARP inhibitors. ACS Chem.Biol. 15 325 PMID: 32017532

If you know of a relevant reference for PARPYnD, please let us know.

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Keywords: PARPYnD, PARPYnD supplier, potent, PARP1, PARP2, PARP6, enzymes, inhibitors, photoaffinity, probes, polymerase, afbp, fluorescent, label, crosslinking, Poly(ADP-ribose), Polymerase, 7410, Tocris Bioscience

Citations for PARPYnD

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