Home / Pharmacology / GPCRs / 7-TM Receptors / Smoothened Receptors / Smoothened Receptor Agonists / SAG dihydrochloride

SAG dihydrochloride

Cat. No. 6390 3 Citations 1 Review Submit a Review Datasheet / COA / SDS
Pricing Availability   Qty
Description: Dihydrochloride salt of SAG; Smo receptor agonist
Chemical Name: 3-Chloro-N-[trans-4-(methylamino)cyclohexyl]-N-[[3-(4-pyridinyl)phenyl]methyl]benzo[b]thiophene-2-carboxamide dihydrochloride
Purity: ≥98% (HPLC)
Datasheet
Citations (3)
Reviews (1)
Literature (1)
Biological Activity Technical Data Solubility Calculators Datasheets References

Biological Activity for SAG dihydrochloride

SAG dihydrochloride is a water-soluble dihydrochloride salt of SAG (Cat. No. 4366). SAG is a potent Smoothened (Smo) receptor agonist (Kd = 59 nM); antagonizes Cyclopamine (Cat. No. 1623) action at the Smo receptor. SAG potently activates the Hedgehog signaling pathway in Shh-light 2 cells (EC50 ~ 3 nM) and induces pathway activation independently of Ptch proteins. SAG is a putative inhibitor of a cellular component required for Hedgehog signaling and also enhances neuronal differentiation of iPSCs into dopaminergic neurons. The compound can be used in protocols for the chemical reprogramming of somatic cells to iPSCs.

Compound Libraries for SAG dihydrochloride

SAG dihydrochloride is also offered as part of the Tocriscreen 2.0 Max. Find out more about compound libraries available from Tocris.

Technical Data for SAG dihydrochloride

M. Wt 562.98
Formula C28H28ClN3OS.2HCl
Storage Store at -20°C
Purity ≥98% (HPLC)
CAS Number 2702366-44-5
PubChem ID 71433770
InChI Key SWBYSYANHQTINY-CZBZHNTRSA-N
Smiles ClC1=C(C(N([C@H]2CC[C@H](NC)CC2)CC3=CC=CC(C4=CC=NC=C4)=C3)=O)SC5=CC=CC=C51.Cl.Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for SAG dihydrochloride

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
water 56.3 100
DMSO 56.3 100

Preparing Stock Solutions for SAG dihydrochloride

The following data is based on the product molecular weight 562.98. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.78 mL 8.88 mL 17.76 mL
5 mM 0.36 mL 1.78 mL 3.55 mL
10 mM 0.18 mL 0.89 mL 1.78 mL
50 mM 0.04 mL 0.18 mL 0.36 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.
=
x
x
g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Dilution Calculator

Calculate the dilution required to prepare a stock solution.
x
=
x

Product Datasheets for SAG dihydrochloride

Certificate of Analysis / Product Datasheet
Select another batch:
Safety Datasheet

References for SAG dihydrochloride

References are publications that support the biological activity of the product.

Guan et al (2022) Chemical reprogramming of human somatic cells to pluripotent stem cells. Nature 605 325 PMID: 35418683

If you know of a relevant reference for SAG dihydrochloride, please let us know.

View Related Products by Target

View Related Products by Product Action

View all Smoothened Receptor Agonists

Keywords: SAG dihydrochloride, SAG dihydrochloride supplier, Potent, Smoothened, Smo, receptors, agonists, activates, Hedgehog, signaling, pathway, activator, enhancers, neuronal, differentiation, iPSCs, induced, pluripotent, stem, cells, dopaminergic, neurons, neurones, 912545-86-9, reprogramming, Receptors, Neural, Stem, Cells, Cell, Reprogramming, 6390, Tocris Bioscience

3 Citations for SAG dihydrochloride

Citations are publications that use Tocris products. Selected citations for SAG dihydrochloride include:

Lucia et al (2020) HDL inhibits endoplasmic reticulum stress-induced apoptosis of pancreatic β-cells in vitro by activation of Smoothened. J Lipid Res 61 492-504 PMID: 31907205

Madeline A et al (2021) Population-scale single-cell RNA-seq profiling across dopaminergic neuron differentiation. Nat Genet 53 304-312 PMID: 33664506

Joaquin M et al (2018) Trisomy 21 Represses Cilia Formation and Function. Dev Cell 46 641-650.e6 PMID: 30100262

Do you know of a great paper that uses SAG dihydrochloride from Tocris? Please let us know.

Reviews for SAG dihydrochloride

Average Rating: 5 (Based on 1 Review.)

5 Star
100%
4 Star
0%
3 Star
0%
2 Star
0%
1 Star
0%

Have you used SAG dihydrochloride?

Submit a review and receive an Amazon gift card.

$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


MUCH better than the others!!.
By Anonymous on 08/28/2020
Assay Type: In Vitro
Species: Human
Cell Line/Tissue: stem cells

This SAG performs the BEST for stem cell differentiation to neuronal lineages. Other brands do not work. We use it at 1uM after dual SMAD inhibition.

Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.

Stem Cell Research Product Guide

Stem Cell Research Product Guide

This product guide provides a background to the use of small molecules in stem cell research and lists over 200 products for use in:

  • Self-renewal and Maintenance
  • Differentiation
  • Reprogramming
  • Organoid Generation
  • GMP and Ancillary Material Grade Products