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Submit ReviewSAHA
Description: Class I and II HDAC inhibitor
Alternative Names: Vorinostat
Chemical Name: N-Hydroxy-N'-phenyloctanediamide
Purity: ≥98% (HPLC)
Biological Activity for SAHA
SAHA inhibits Class I and II histone deacetylases (HDACs); induces accumulation of acetylated histones H2A, H2B, H3 and H4 in transformed cultured cells. Suppresses cell growth in a range of cancer cell lines; induces apoptosis in cutaneous T cell lymphoma cells in vitro. Activates autophagy. SAHA increases efficiency of transcription factor-induced reprogramming of mouse embryonic fibroblasts (MEF) to induced pluripotent stem cells (iPSC). Enhances adeno-associated virus transduction of HeLa cells. Also induces activation and replication of latent HIV in vitro.
Compound Libraries for SAHA
SAHA is also offered as part of the Tocriscreen 2.0 Max, Tocriscreen Antiviral Library, Tocriscreen Epigenetics Library, Tocriscreen FDA-Approved Drugs and Tocriscreen Stem Cell Library. Find out more about compound libraries available from Tocris.
Technical Data for SAHA
| M. Wt | 264.32 |
| Formula | C14H20N2O3 |
| Storage | Store at -20°C |
| Purity | ≥98% (HPLC) |
| CAS Number | 149647-78-9 |
| PubChem ID | 5311 |
| InChI Key | WAEXFXRVDQXREF-UHFFFAOYSA-N |
| Smiles | O=C(CCCCCCC(NO)=O)NC1=CC=CC=C1 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for SAHA
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 26.43 | 100 |
Preparing Stock Solutions for SAHA
The following data is based on the product molecular weight 264.32. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 3.78 mL | 18.92 mL | 37.83 mL |
| 5 mM | 0.76 mL | 3.78 mL | 7.57 mL |
| 10 mM | 0.38 mL | 1.89 mL | 3.78 mL |
| 50 mM | 0.08 mL | 0.38 mL | 0.76 mL |
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Product Datasheets for SAHA
References for SAHA
References are publications that support the biological activity of the product.
Butler et al (2000) Suberoylanilide hydroxamic acid, an inhibitor of histone deacetylase, suppresses the growth of prostate cancer cells in vitro and in vivo. Cancer Res. 60 5165 PMID: 11016644
Leoni et al (2002) The antitumor histone deacetylase inhibitor suberoylanilide hydroxamic acid exhibits antiinflammatory properties via suppression of cytokines. Proc.Natl.Acad.Sci.U.S.A 99 2995 PMID: 11867742
Marks and Breslow (2007) Dimethyl sulfoxide to vorinostat: development of this histone deacetylase inhibitor as an anticancer drug. Nat.Biotechnol. 25 84 PMID: 17211407
Huangfu et al (2008) Induction of pluripotent stem cells by defined factors is greatly improved by small-molecule compounds. Nat.Biotechnol. 26 795 PMID: 18568017
Galluzzi et al (2017) Pharmacological modulation of autophagy: therapeutic potential and persisting obstacles. Nat.Rev.Drug.Discov. 16 487 PMID: 28529316
Nicolson et al (2016) Identification and validation of small molecules that enhance recombinant adeno-associated virus transduction following high-throughput screens. J.Virol. 90 7019 PMID: 27147738
Narasipura et al (2014) Epigenetic regulation of HIV-1 latency in astrocytes. J.Virol. 88 3031 PMID: 24352441
If you know of a relevant reference for SAHA, please let us know.
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Keywords: SAHA, SAHA supplier, histone, deacetylases, HDACs, inhibits, inhibitors, Class, I, II, vorinostat, anticancer, antiproliferatives, epigenetics, proapoptotic, viral, transduction, enhancer, enhances, stem, cell, reporgramming, Vorinostat, Autophagy, Non-selective, Non-Selective, Viral, Transduction, Enhancers, Stem, Cell, Reprogramming, 4652, Tocris Bioscience
8 Citations for SAHA
Citations are publications that use Tocris products. Selected citations for SAHA include:
Ng-Blichfeldt et al (2018) Retinoic acid signaling balances adult distal lung epithelial progenitor cell growth and differentiation. EBioMedicine 36 461 PMID: 30236449
Bhattacharya et al (2017) RAD51 interconnects between DNA replication, DNA repair and immunity. Nucleic Acids Res 45 4590 PMID: 28334891
Steven L et al (2022) INF2-mediated actin filament reorganization confers intrinsic resilience to neuronal ischemic injury. Nat Commun 13 6037 PMID: 36229429
Jacques et al (2022) Microtubules restrict F-actin polymerization to the immune synapse via GEF-H1 to maintain polarity in lymphocytes. Elife 11 PMID: 36111670
Nianli et al (2019) Compounds targeting class II histone deacetylases do not cause panHDACI-associated impairment of megakaryocyte differentiation. Exp Hematol 72 36-46 PMID: 30611870
Sarenac et al (2016) Single-cell analysis reveals IGF-1 potentiation of inhibition of the TGF-β/Smad pathway of fibrosis in humankeratocytes in vitro Scientific Reports 6 34373 PMID: 27687492
Asgerally T et al (2020) ARID1A Mutations Promote P300-Dependent Endometrial Invasion through Super-Enhancer Hyperacetylation. Cell Rep 33 108366 PMID: 33176148
Gergely et al (2020) Celecoxib Prevents Doxorubicin-Induced Multidrug Resistance in Canine and Mouse Lymphoma Cell Lines. Cancers (Basel) 12 PMID: 32365663
Do you know of a great paper that uses SAHA from Tocris? Please let us know.
Reviews for SAHA
Average Rating: 5 (Based on 1 Review.)
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Excellent histone deacetylase inhibitor.
By
Luiz Alexandre Magno on 11/25/2021
Assay Type: In Vitro
Species: Rat
Cell Line/Tissue: PC12
Our lab uses this drug to monitor transcriptional activity of genes that depend on class I, II and IV of histone deacetylase. In the experiment shown in the figure, we have treated PC12 cells with 3 different concentrations of SAHA for 3h. We found increased transcriptional activity of PI3K gene upon SAHA treatment in a dose-dependent manner.
SAHA can be easily resuspended in DMSO. Make a stock solution with a concentration that will not exceed 0.01% of DMSO in the cell culture to avoid toxicity.
Literature in this Area
Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!
*Please note that Tocris will only send literature to established scientific business / institute addresses.
Epigenetics Scientific Review
Written by Susanne Müller-Knapp and Peter J. Brown, this review gives an overview of the development of chemical probes for epigenetic targets, as well as the impact of these tool compounds being made available to the scientific community. In addition, their biological effects are also discussed. Epigenetic compounds available from Tocris are listed.
Rheumatoid Arthritis Poster
Rheumatoid arthritis (RA) is a chronic destructive inflammatory autoimmune disease that results from a breakdown in immune tolerance, for reasons that are as yet unknown. This poster summarizes the pathology of RA and the inflammatory processes involved, as well as describing some of the epigenetic modifications associated with the disease and the potential for targeting these changes in the discovery of new treatments.