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Sal 003

Cat. No. 3657 5 Citations Submit a Review Datasheet / COA / SDS
Pricing Availability   Qty
Description: Cell-permeable inhibitor of eIF2α dephosphorylation
Chemical Name: 3--Phenyl-N-(2,2,2-trichloro-1-((((4-chlorophenyl)amino)carbonothioyl)amino)ethyl)acrylamide
Purity: ≥99% (HPLC)
Datasheet
Citations (5)
Reviews
Biological Activity Technical Data Solubility Calculators Datasheets References

Biological Activity for Sal 003

Sal 003 is a cell-permeable inhibitor of cellular phosphatase complexes that dephosphorylate eukaryotic translation initiation factor 2 subunit α (eIF2α). Analog of salubrinal (Cat. No. 2347) with improved aqueous solubility. Shown to prevent the induction of hippocampal long-term potentiation (LTP) and memory formation (LTM) in mice.

Compound Libraries for Sal 003

Sal 003 is also offered as part of the Tocriscreen 2.0 Max and Tocriscreen Epigenetics Library. Find out more about compound libraries available from Tocris.

Technical Data for Sal 003

M. Wt 463.21
Formula C18H15Cl4N3OS
Storage Store at -20°C
Purity ≥99% (HPLC)
CAS Number 1164470-53-4
PubChem ID 5717737
InChI Key TVNBASWNLOIQML-IZZDOVSWSA-N
Smiles S=C(NC(NC(/C=C/C2=CC=CC=C2)=O)C(Cl)(Cl)Cl)NC1=CC=C(Cl)C=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for Sal 003

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 46.32 100

Preparing Stock Solutions for Sal 003

The following data is based on the product molecular weight 463.21. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.16 mL 10.79 mL 21.59 mL
5 mM 0.43 mL 2.16 mL 4.32 mL
10 mM 0.22 mL 1.08 mL 2.16 mL
50 mM 0.04 mL 0.22 mL 0.43 mL

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References for Sal 003

References are publications that support the biological activity of the product.

Raven et al (2008) PKR and PKR-like endoplasmic reticulum kinase induce the protease-dependent degradation of cyclin D1 via a mechanism requiring eukaryotic initiation factor 2α phosphorylation. J.Biol.Chem. 283 3097 PMID: 18063576

Baltiz et al (2007) The eIF2α kinases PERK and PKR activate glucogen synthase kinase 3 to promote the proteasomal degradation of p53. J.Biol.Chem. 282 31675 PMID: 17785458

Costa-Mattioli et al (2007) eIF2α phosphorylation bidirectionally regulates the switch from short- to long-term synaptic plasticity and memory. Cell 129 195 PMID: 17418795

If you know of a relevant reference for Sal 003, please let us know.

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5 Citations for Sal 003

Citations are publications that use Tocris products. Selected citations for Sal 003 include:

Jing et al (2021) Adaptive translational pausing is a hallmark of the cellular response to severe environmental stress. Mol Cell 81 4191-4208.e8 PMID: 34686314

Jing et al (2022) Stress-induced perturbations in intracellular amino acids reprogram mRNA translation in osmoadaptation independently of the ISR. Cell Rep 40 111092 PMID: 35858571

Albert S et al (2022) Integrated Stress Response Regulation of Corneal Epithelial Cell Motility and Cytokine Production. Invest Ophthalmol Vis Sci 63 1 PMID: 35802384

Krokowski (2017) GADD34 function in protein trafficking promotes adaptation to hyperosmotic stress in human corneal cells. Cell Rep 21 2895 PMID: 29212034

Do you know of a great paper that uses Sal 003 from Tocris? Please let us know.

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