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Submit ReviewTFAX 546, SE
Description: Yellow fluorescent dye; amine reactive
Chemical Name: 6-[2-Carboxy-3,4,6-trichloro-5-[[2-[[6-[(2,5-dioxo-1-pyrrolidinyl)oxy]-6-oxohexyl]amino]-2-oxoethyl]thio]phenyl]-1,2,3,4,8,9,10,11-octahydro-2,2,4,8,10,10-hexamethyl-12,14-disulfopyrano[3,2-g:5,6-g']diquinolin-13-ium triethylammonium salt
Biological Activity for TFAX 546, SE
TFAX 546, SE is an amine reactive yellow fluorescent dye. Insensitive to pH in the range 4 - 10. Forms bright and photostable conjugates with proteins and antibodies. Suitable for use in microscopy. Excitation maximum = 554 nm; emission maximum = 570 nm; extinction coefficient = 112,000 M-1cm-1; quantum yield = 0.79.
Optical Data for TFAX 546, SE
| Emission Color | Yellow |
|---|---|
| Brightness | |
| λabs | 554 nm |
| λem | 570 nm |
| Extinction Coefficient (ε) | 112000 M-1cm-1 |
| Quantum Yield (φ) | 0.79 |
| Closest Laser line | 532 nm |
| Reactive Group | NHS ester |
| Reactivity | Primary amines |
| Cell Permeable | No |
| pH Sensitivity | pH insensitive pH 4-10 |
| Application | microscopy |
| Alternative For | TMR, Cyanine3, Alexa Fluor 546 SE, AF 546 SE |
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company.
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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Technical Data for TFAX 546, SE
| M. Wt | 1159.6 |
| Formula | C44H47Cl3N4O14S.(CH3CH2)3NH |
| Storage | Store at -20°C |
| Smiles | ClC(C(Cl)=C1C(O)=O)=C(SCC(NCCCCCC(ON2C(CCC2=O)=O)=O)=O)C(Cl)=C1C(C3=CC4=C(NC(C)(C)CC4C)C(S(=O)([O-])=O)=C3O5)=C6C5=C(S(=O)([O-])=O)C7=[NH+]C(C)(C)CC(C)C7=C6.CC[NH+](CC)CC |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Product Datasheets for TFAX 546, SE
References for TFAX 546, SE
References are publications that support the biological activity of the product.
Hone et al (2010) Alexa Fluor 546-ArIB[V11L;V16A] is a potent ligand for selectively labeling α 7 nicotinic acetylcholine receptors. J.Neurochem. 114 994 PMID: 20492354
Iwata et al (2005) Increased susceptibility of cytoplasmic over nuclear polyglutamine aggregates to autophagic degradation. Proc.Natl.Acad.Sci.USA. 102 13135 PMID: 16141322
Panchuk-Voloshina et al (1999) Alexa dyes, a series of new fluorescent dyes that yield exceptionally bright, photostable conjugates. J.Histochem.Cytochem. 47 1179 PMID: 10449539
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Fluorescent Dyes and Probes Research Product Guide
This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:
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