Home / Cell Biology / Cytoskeleton & Motor Proteins / Actin / Actin Inhibitors / Wiskostatin

Wiskostatin

Cat. No. 4434 3 Citations Submit a Review Datasheet / COA / SDS
Pricing Availability   Qty
Description: N-WASP inhibitor; inhibits Arp2/3 activation
Chemical Name: 3,6-Dibromo-α-[(dimethylamino)methyl]-9H-cabazole-9-ethanol
Purity: ≥99% (HPLC)
Datasheet
Citations (3)
Reviews
Biological Activity Technical Data Solubility Calculators Datasheets References

Biological Activity for Wiskostatin

Wiskostatin is an inhibitor of neural Wiskott-Aldrich syndrome protein (N-WASP) activity. Interacts with the regulatory GTPase-binding domain of N-WASP; inhibits activation of Arp2/3 complex by maintaining N-WASP in an inactive conformation. Also inhibits PIP2-induced actin polymerization (EC50 ~ 4μM).

Compound Libraries for Wiskostatin

Wiskostatin is also offered as part of the Tocriscreen 2.0 Max. Find out more about compound libraries available from Tocris.

Technical Data for Wiskostatin

M. Wt 426.15
Formula C17H18Br2N2O
Storage Store at +4°C
Purity ≥99% (HPLC)
CAS Number 253449-04-6
PubChem ID 2775510
InChI Key XUBJEDZHBUPBKL-UHFFFAOYSA-N
Smiles BrC(C=C3)=CC1=C3N(CC(O)CN(C)C)C2=C1C=C(Br)C=C2

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for Wiskostatin

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 42.62 100

Preparing Stock Solutions for Wiskostatin

The following data is based on the product molecular weight 426.15. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.35 mL 11.73 mL 23.47 mL
5 mM 0.47 mL 2.35 mL 4.69 mL
10 mM 0.23 mL 1.17 mL 2.35 mL
50 mM 0.05 mL 0.23 mL 0.47 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.
=
x
x
g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Dilution Calculator

Calculate the dilution required to prepare a stock solution.
x
=
x

References for Wiskostatin

References are publications that support the biological activity of the product.

Wegner et al (2008) N-WASP and the Arp2/3 complex are critical regulators of actin in the development of dendritic spines and synapses. J.Biol.Chem. 283 15912 PMID: 18430734

Peterson et al (2004) Chemical inhibition of N-WASP by stabilization of a native autoinhibited conformation. Nat.Struct.Mol.Biol. 11 747 PMID: 15235593

Guerriero and Weisz et al (2006) N-WASP inhibitor wiskostatin nonselectively perturbs membrane transport by decreasing cellular ATP levels. Am.J.Physiol.Cell Physiol. 292 C1562 PMID: 17092993

If you know of a relevant reference for Wiskostatin, please let us know.

View Related Products by Target

View Related Products by Product Action

View all Actin Inhibitors

Keywords: Wiskostatin, Wiskostatin supplier, Arp2/3, complex, n-wasp, wiskott-aldrich, syndrome, inhibitors, inhibits, actin, polymerization, 3,6-Dibromo-a-[(dimethylamino)methyl]-9H-cabazole-9-ethanol, Actin, 4434, Tocris Bioscience

3 Citations for Wiskostatin

Citations are publications that use Tocris products. Selected citations for Wiskostatin include:

Raymond P et al (2022) Identification of a pharmacological approach to reduce ACE2 expression and development of an in vitro COVID-19 viral entry model. J Virus Erad 8 100307 PMID: 36514715

Rassokhin and Pakhomov (2014) Cellular regulation of extension and retraction of pseudopod-like blebs produced by nanosecond pulsed electric field (nsPEF). Cell Biochem Biophys 69 555 PMID: 24488232

Yogaditya et al (2021) A cryo-electron tomography workflow reveals protrusion-mediated shedding on injured plasma membrane. Sci Adv 7 PMID: 33771860

Do you know of a great paper that uses Wiskostatin from Tocris? Please let us know.

Reviews for Wiskostatin

There are currently no reviews for this product. Be the first to review Wiskostatin and earn rewards!

Have you used Wiskostatin?

Submit a review and receive an Amazon gift card.

$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review