Janelia Fluor^® Dyes

Janelia Fluor^® (JF) Dye Key Features and Applications

Engineered for enhanced brightness and longer fluorescence lifetime, Janelia Fluor^® (JF) dyes offer significant advantages over spectrally similar dyes and incorporate additional properties for application in specific super-resolution microscopy techniques.

Janelia Fluor^® (JF) Dyes are:

• Exceptionally Bright
• Cell Permeable
• Highly Photostable
• Supplied with a choice of reactive groups for biomolecule conjugation
• Able to be converted to relevant substrate for use in self-labeling tag systems, e.g. HaloTag^® and SNAP-tag^®

HaloTag is a trademark of Promega Corporation, and SNAP-tag is a trademark of New England BioLabs, Inc.

Janelia Fluor^® (JF) Dyes Key Applications:

• Suitable for use in confocal microscopy, IHC, ICC and flow cytometry
• Especially well-suited to live-cell imaging
• Ideal for super-resolution techniques including STED and dSTORM
• Photoactivatable Janelia Fluor^® dyes are compatible with PALM microscopy

Figure 1: Application of Janelia Fluor^® Dyes in Cardiac Tissue. Super-resolution image (dSTORM) of muscle tissue from the left ventricle of an adult pig heart. Left: JF 646 (Cat. No. 6148) was used to label collagen VI, which is present on the cell plasma membrane, and imaged with a 642 nm excitation laser. A clearer structure is achieved in comparison with the widefield image (top right). Scale bar 2 μm. Right: - Displays the distribution of collagen VI in the interstitial space between muscle cells. Labeled with a primary ab against collagen VI and a secondary ab conjugated to JF 549 (Cat. No. 6147). Scale: 50 μm.

Images kindly provided by Prof. Christian Soeller, University of Exeter; acquired by Alex Clowsley and Anna Meletiou.

Janelia Fluor^® Cellular Probes

Tocris has an exclusive range of Janelia Fluor^® dye-based probes, for labeling cellular targets. These include Taxol Janelia Fluor^® 646 (Cat. No. 6266), a fluorogenic probe for microtubule staining, which is non-fluorescent until bound to microtubules, enabling no-wash experiments (protocol available). BOP-JF646 (Cat. No. 6997), a fluorogenic red fluorescent dual α₉β₁/α₄β₁ integrins inhibitor, which is exceptionally photostable and BOP-JF549 (Cat. No. 6996), a yellow fluorogenic fluorescent dual α₉β₁/α₄β₁ integrin inhibitor. All three of these probes are fluorogenic, meaning they fluoresce only when bound to their target, allowing for hassle-free no-wash experiments, with bright, low noise signals, perfect for sensitive or low expression experiments.

Figure 2: Application of Janelia Fluor^® Probes Left: COS7 cells were labeled with 3 μM Taxol Janelia Fluor^® 646 (Cat. No. 6266) for one hour at 37°C. Images were taken on a Leica TCS SP8 Confocal Laser Scanning Microscope. Image kindly provided by Prof. Christian Soeller, University of Exeter; acquired by Evelina Lucinskaite, Anna Meletiou and Alexander Clowsley. Middle: Immortalized human keratinocyte cells expressing a GFP-tagged α₄ integrin were treated with 10 μM BOP-Janelia Fluor^® 646 (Cat. No. 6997). Cells were incubated at 37°C and imaged using a Nikon A1R confocal microscope. Scale bar = 10 μm. Right: Immortalized human keratinocytes expressing a GFP-tagged α₄ integrin were incubated with 10 μm BOP-Janelia Fluor^® 549 (Cat. No. 6996). Cells were incubated at 37°C and imaged using Nikon A1R confocal microscope. Scale bar = 10 μm. Middle and right images kindly provided by Willow Hight-Warburton, Kings College London.