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Submit ReviewBOP-JF549
Description: Yellow fluorescent dual α9β1/α4β1 integrin inhibitor comprising BOP conjugated to Janelia Fluor® 549; fluorogenic; photostable
Chemical Name: 2-(3-(Azetidin-1-ium-1-ylidene)-6-(azetidin-1-yl)-3H-xanthen-9-yl)-4-(((1-((3R,5S)-5-(((S)-1-carboxy-2-(4-((pyrrolidine-1-carbonyl)oxy)phenyl)ethyl)carbamoyl)-1-(phenylsulfonyl)pyrrolidin-3-yl)-1H-1,2,3-triazol-4-yl)methyl)carbamoyl)benzoate
Purity: ≥85% (HPLC)
Biological Activity for BOP-JF549
BOP-JF549 is a fluorescent dual α9β1/α4β1 integrin inhibitor. Comprises BOP (Cat. No. 6047) conjugated to Janelia Fluor® 549 (Cat. No. 6147). Fluorogenic: fluoresces only once bound to integrins, enabling hassle-free no-wash experiments. Bright and photostable, enabling live cell tracking of integrin receptors over long time-course experiments. Excitation maximum = 549 nm; emission maximum = 571 nm.
Scientific Data
Application of BOP-JF646 in Immortalized Human Keratinocyte Cells Immortalized human keratinocyte cells expressing a GFP-tagged alpha 4 integrin were treated with 10 μM BOP-Janelia Fluor® 646. Cells were incubated at 37°C and imaged using a Nikon A1R confocal microscope. Scale bar = 10 μm. Image kindly provided by Willow Hight-Warburton, Kings College London.
Optical Data for BOP-JF549
| Emission Color | Yellow |
|---|---|
| Brightness | |
| λabs | 549 nm |
| λem | 571 nm |
| Closest Laser line | 561 nm |
| Application | Flow Cytometry, Confocal Microscopy, Super Resolution Microscopy (SRM) |
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectral Viewer
Technical Data for BOP-JF549
| M. Wt | 1048.14 |
| Formula | C55H53N9O11S |
| Storage | Store at -20°C |
| Purity | ≥85% (HPLC) |
| InChI Key | CKYXWZFUNXSZMN-AYQWXEKZSA-N |
| Smiles | OC([C@H](CC1=CC=C(OC(N2CCCC2)=O)C=C1)NC([C@@H]3C[C@H](CN3S(=O)(C4=CC=CC=C4)=O)N5C=C(N=N5)CNC(C6=CC=C(C(C(C7=CC=C(C=C7OC8=C/9)N%10CCC%10)=C8C=CC9=[N+]%11CCC/%11)=C6)C([O-])=O)=O)=O)=O |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Product Datasheets for BOP-JF549
References for BOP-JF549
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Keywords: BOP-JF549, BOP-JF549 supplier, Yelllow, fluorescent, dual, α9β1/α4β1, integrins, inhibitors, BOP, labels, stains, HSC, progenitors, hematopoietic, stem, cells, Stem, Cell, Signaling, Integrins, Fluorescent, Integrin, Probes, Hematopoietic, Cells, 6996, Tocris Bioscience
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Fluorescent Dyes and Probes Research Product Guide
This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:
- Fluorescent Dyes, including dyes for flow cytometry
- Fluorescent Probes and Stains, including our new MitoBrilliantTM mitochondria stains
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