7-Aminoactinomycin D

Cat. No. 7121 提交评论说明书 / 分析证书（CoA） / 化学品安全技术说明书（SDS）

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说明： Red-fluorescent DNA stain, membrane impermeant to live cells. Used for: apoptosis detection, cell viability staining. Application: flow cytometry, fluorescence microscopy

化学名： 2,7-Diamino-N¹,N⁹-bis(6,13-diisopropyl-2,5,9-trimethyl-1,4,7,11,14-pentaoxohexadecahydro-1H-pyrrolo[2,1-i][1]oxa[4,7,10,13]tetraazacyclohexadecin-10-yl)-4,6-dimethyl-3-oxo-3H-phenoxazine-1,9-dicarboxamide

纯度： ≥97% (HPLC)

文献 (1)

生物活性技术数据溶解性计算器说明书参考文献

生物活性 for 7-Aminoactinomycin D

Key information: 7-aminoactinomycin D (7-AAD) is a red-fluorescent DNA stain. It is membrane impermeant to live cells but penetrates dead or damaged cells.

Used for: cell cycle studies, selective detection of dead cells by flow cytometry, chromosome banding analysis, cell sorting, and apoptosis quantification.

Application: fluorescence microscopy and flow cytometry.

Properties and Photophysical Data: G-C base-specific DNA intercalator that forms stable complexes with DNA. May be used in combination with FITC (Cat. No. 5440) or phycoerythrin (PE). Fluorescent analogue of actinomycin D (Cat. No. 1229). Can be excited using 488 nm (blue), 532 nm (green), and 561 nm (yellow-green) laser lines. Suitable for multicolor fluorescence microscopy experiments. Excitation and emission maxima (λ) are 549 nm and 655 nm, respectively.

Optical Data for 7-Aminoactinomycin D

λ_abs	549 nm
λ_em	655 nm
Application	Fluorescence Microscopy, Flow Cytometry

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技术数据 for 7-Aminoactinomycin D

分子量	1270.45
公式	C₆₂H₈₇N₁₃O₁₆
储存	Store at -20°C
纯度	≥97% (HPLC)
CAS Number	7240-37-1
PubChem ID	14924508
InChI Key	YXHLJMWYDTXDHS-UHFFFAOYSA-N
Smiles	NC1=C(C(NC(C(C)OC(C(C(C)C)N(C(CN(C)C2=O)=O)C)=O)C(NC(C(C)C)C(N3C2CCC3)=O)=O)=O)C4=NC5=C(C(NC(C(NC(C(C)C)C6=O)=O)C(C)OC(C(C(C)C)N(C(CN(C)C(C7N6CCC7)=O)=O)C)=O)=O)C=C(N)C(C)=C5OC4=C(C)C1=O

上方提供的技术数据仅供参考。批次相关数据请参见分析证书。

Tocris products are intended for laboratory research use only, unless stated otherwise.

溶解性数据 for 7-Aminoactinomycin D

	溶剂	最高浓度 mg/mL	最高浓度 mM
溶解性
溶解性	DMSO	2	2

制备储备液 for 7-Aminoactinomycin D

以下数据基于产品分子量 1270.45。 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

浓度/溶剂体积/质量	1 mg	5 mg	10 mg
0.02 mM	39.36 mL	196.78 mL	393.56 mL
0.1 mM	7.87 mL	39.36 mL	78.71 mL
0.2 mM	3.94 mL	19.68 mL	39.36 mL
1 mM	0.79 mL	3.94 mL	7.87 mL

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产品说明书 for 7-Aminoactinomycin D

分析证书/产品说明书

化学品安全技术说明书

参考文献 for 7-Aminoactinomycin D

参考文献是支持产品生物活性的出版物。

Zelenin et al (1984) 7-Amino-actinomycin D as a specific fluorophore for DNA content analysis by laser flow cytometry. Cytometry 5 348 PMID: 6468175

Schmid et al (1992) Dead cell discrimination with 7-amino-actinomycin D in combination with dual color immunofluorescence in single laser flow cytometry. Cytometry 13 204 PMID: 1547670

Philpott et al (1996) The use of 7-amino actinomycin D in identifying apoptosis: Simplicity of use and broad spectrum of application compared with other techniques. Blood 87 2244 PMID: 8630384

If you know of a relevant reference for 7-Aminoactinomycin D, please let us know.

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