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Submit ReviewActinomycin D
说明： Inhibits RNA polymerase
化学名： 2-Amino-(N,N)-1-bis(hexadecahydro-6,13-diisopropyl-2,5,9-trimethyl-1,4,7,11,14-pentaoxo-1H-pyrrolo[2,1]-[1,4,7,10,13] oxatetraazacyclohexadecin-10-yl)-4,6-dimethyl-3-oxo-3H-phenoxazine-1,9-dicarboxamide
纯度： ≥95% (HPLC)
生物活性 for Actinomycin D
Actinomycin D is an anti-neoplastic antibiotic. Inhibits RNA polymerase and is a potent inducer of apoptosis.
技术数据 for Actinomycin D
| 分子量 | 1255.43 |
| 公式 | C62H86N12O16 |
| 储存 | Store at -20°C |
| 纯度 | ≥95% (HPLC) |
| CAS Number | 50-76-0 |
| PubChem ID | 2019 |
| InChI Key | RJURFGZVJUQBHK-DCUCITLNSA-N |
| Smiles | [H][C@@]12CCCN1C([C@H](C(C)C)NC([C@H]([C@H](OC([C@@H](N(C(CN(C2=O)C)=O)C)C(C)C)=O)C)NC(C3=C4N=C5C(OC4=C(C=C3)C)=C(C(C(N)=C5C(N[C@H]6[C@H](OC([C@@H](N(C(CN(C([C@@]7(CCCN7C([C@H](C(C)C)NC6=O)=O)[H])=O)C)=O)C)C(C)C)=O)C)=O)=O)C)=O)=O)=O |
上方提供的技术数据仅供参考。批次相关数据请参见分析证书。
Tocris products are intended for laboratory research use only, unless stated otherwise.
溶解性数据 for Actinomycin D
| 溶剂 | 最高浓度 mg/mL | 最高浓度 mM | |
|---|---|---|---|
| 溶解性 | |||
| DMSO | 62.77 | 50 |
制备储备液 for Actinomycin D
以下数据基于产品分子量 1255.43。 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| 浓度/溶剂体积/质量 | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.5 mM | 1.59 mL | 7.97 mL | 15.93 mL |
| 2.5 mM | 0.32 mL | 1.59 mL | 3.19 mL |
| 5 mM | 0.16 mL | 0.8 mL | 1.59 mL |
| 25 mM | 0.03 mL | 0.16 mL | 0.32 mL |
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产品说明书 for Actinomycin D
参考文献 for Actinomycin D
参考文献是支持产品生物活性的出版物。
Aktipis et al (1981) A kinetic study on the mechanism of inhibition of RNA synthesis catalyzed by DNA-dependent RNA polymerase. Differences in inhibition by ethidium bromide, 3,8-diamino-6-ethylphenanthridinium bromide and actinomycin D. Biochim.Biophys.Acta 655 278 PMID: 7025910
Glynn et al (1992) Apoptosis induced by actinomycin D, camptothecin or aphidicolin can occur in all phases of the cell cycle. Biochem.Soc.Trans. 20 84S PMID: 1634006
Jeeninga et al (1998) The mechanism of actinomycin D-mediated inhibition of HIV-1 reverse transcription. Nucleic Acids Res. 26 5472 PMID: 9826774
If you know of a relevant reference for Actinomycin D, please let us know.
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关键词: Actinomycin D, Actinomycin D supplier, RNA, polymerases, inhibitors, inhibits, Antineoplastic, antibiotics, Apoptosis, Inducers, ActinomycinD, DNA,, and, Protein, Synthesis, Antibiotics, Polymerase, 1229, Tocris Bioscience
⚠ WARNING: This product can expose you to chemicals including Actinomycin D, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov
19 篇 Actinomycin D 的引用文献
引用文献是使用了 Tocris 产品的出版物。 Actinomycin D 的部分引用包括：
Livingstone et al (2019) Secreted Amyloid Precursor Protein-Alpha Promotes Arc Protein Synthesis in Hippocampal Neurons. Front Mol Neurosci 12 198 PMID: 31474829
Yuan and Burrell (2013) Endocannabinoid-dependent long-term depression in a nociceptive synapse requires coordinated presynaptic and postsynaptic transcription and translation. J Neurosci 33 4349 PMID: 23467351
Huang et al (2010) Pharmacological and genetic accumulation of hypoxia-inducible factor-1alpha enhances excitatory synaptic transmission in hippocampal neurons through the production of vascular endothelial growth factor. J Neurosci 30 6080 PMID: 20427666
Di et al (2016) Acute Stress Suppresses Synaptic Inhibition and Increases Anxiety via Endocannabinoid Release in the Basolateral Amygdala. J.Neurosci. 36 8461 PMID: 27511017
Kang et al (2012) Loss of neuron-astroglial interaction rapidly induces protective CNTF expression after stroke in mice. J Neurosci 32 9277 PMID: 22764235
Tian et al (2010) MEF-2 regulates activity-dependent spine loss in striatopallidal medium spiny neurons. Mol Cell Neurosci 44 94 PMID: 20197093
Visavadiya (2016) Integrin-FAK signaling rapidly and potently promotes mitochondrial function through STAT3. Cell Communication and Signaling 14 32 PMID: 27978828
Parker and Kooi (2014) Microplate-based screening for small molecule inhibitors of neuropilin-2/vascular endothelial growth factor-C interactions. J Biol Chem 453 42525 PMID: 24583243
Cheang et al (2011) Endothelial nitric oxide synthase enhancer reduces oxidative stress and restores endothelial function in db/db mice. Biochem Biophys Res Commun 92 267 PMID: 21875904
Wang et al (2014) SynGAP regulates protein synthesis and homeostatic synaptic plasticity in developing cortical networks. PLoS One 8 e83941 PMID: 24391850
Gutierrez et al (2014) The anticancer agent di-2-pyridylketone 4,4-dimethyl-3-thiosemicarbazone (Dp44mT) overcomes prosurvival autophagy by two mechanisms: persistent induction of autophagosome synthesis and impairment of lysosomal integrity. J Biol Chem 289 33568 PMID: 25301941
Khattar et al (2019) Structural determinants and genetic modifications enhance BMP2 stability and extracellular secretion. FASEB Bioadv 1 180 PMID: 31225515
Podszywalow-Bartnicka et al (2014) Downregulation of BRCA1 protein in BCR-ABL1 leukemia cells depends on stress-triggered TIAR-mediated suppression of translation. Cardiovasc Res 13 3727 PMID: 25483082
Wong et al (2009) Cyclooxygenase-2-derived prostaglandin F2alpha mediates endothelium-dependent contractions in the aortae of hamsters with increased impact during aging. Circ Res 104 228 PMID: 19096033
Backlund et al (2009) Posttranscriptional regulation of angiotensin II type 1 receptor expression by glyceraldehyde 3-phosphate dehydrogenase. Nucleic Acids Res 37 2346 PMID: 19246543
Thomson et al (2017) Cell-Type-Specific Translation Profiling Reveals a Novel Strategy for Treating Fragile X Syndrome. Neuron 95 550 PMID: 28772121
Chan et al (2007) Heat shock protein 60 or 70 activates nitric-oxide synthase (NOS) I- and inhibits NOS II-associated signaling and depresses the mitochondrial apoptotic cascade during brain stem death. Cell Cycle 282 4585 PMID: 17150954
Vecsey et al (2007) Histone deacetylase inhibitors enhance memory and synaptic plasticity via CREB:CBP-dependent transcriptional activation. J Neurosci 27 6128 PMID: 17553985
Paukku et al (2008) p100 increases AT1R expression through interaction with AT1R 3'-UTR. Nucleic Acids Res 36 4474 PMID: 18603592
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Mitochondrial bioenergetics measurements by reagent screen.
By
Anonymous on 03/15/2020
分析类型： In Vitro
种属： Mouse
细胞系/组织： mouse brain endothelioma cell line
0.3 μg/ml
In vivo assay.
By
Anonymous on 01/07/2018
分析类型： In Vitro
种属： Human
I used in my experiment for muHL6b cells with 0.2 μg/ml actinomycin D final concentration.