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BDY FL Staurosporine

Cat. No. 7985 提交评论 说明书 / 分析证书（CoA） / 化学品安全技术说明书（SDS）
Pricing Availability   Qty
说明： Fluorescent probe targeting kinases; kinase tracer for TR-FRET assays
纯度： ≥95% (HPLC)
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生物活性 技术数据 溶解性 计算器 说明书 参考文献

生物活性 for BDY FL Staurosporine

BDY FL Staurosporine is a fluorescent kinase-targeting probe. It can be paired with antibodies labelled with TR-FRET donors such as CoraFluor™ 1 (Cat. No. 7920) for high throughput binding and protein quantification assays. BDY FL Staurosporine shows high specificity for tyrosine kinase (TK) and tyrosine kinase-like (TKL) family kinases. Excitation and emission maxima (λ) are 514 and 516 nm, respectively; quantum yield = 0.11; extinction coefficient = 35,000 M-1cm-1.

The measured KD values, from TR-FRET based saturation binding assay data, are provided in the accompanying supplementary file (see below).

Supplementary Information for BDY FL Staurosporine

The measured KD values, from TR-FRET based saturation binding assay data, are provided in the accompanying supplementary file.

Optical Data for BDY FL Staurosporine

BDY FL Staurosporine Dye Spectra
Emission Color Green
Brightness
λabs 514 nm
λem 516 nm
Extinction Coefficient (ε) 35400 M-1cm-1
Quantum Yield (φ) 0.11
Closest Laser line 488 nm
Application Time-resolved fluorescence energy transfer (TR-FRET)

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectral Viewer

Spectral Viewer

技术数据 for BDY FL Staurosporine

分子量 901.82
公式 C49H54BF2N7O7
储存 Store at -20°C
纯度 ≥95% (HPLC)
CAS Number 2411845-57-1
PubChem ID 155531579
InChI Key NXSJMRCTGYTSTK-HKTVIYRYSA-N
Smiles C[C@]12N3C4=C(C5=CC=CC=C53)C(CNC6=O)=C6C7=C4N([C@](C[C@H]([C@H]2OC)NCCOCCOCCOCCNC(CCC8=CC=C(C=C9[N+]%10=C(C=C9C)C)N8[B-]%10(F)F)=O)([H])O1)C%11=CC=CC=C7%11

上方提供的技术数据仅供参考。批次相关数据请参见分析证书。

Tocris products are intended for laboratory research use only, unless stated otherwise.

溶解性数据 for BDY FL Staurosporine

溶剂 最高浓度 mg/mL 最高浓度 mM
溶解性
DMSO 9.02 10

制备储备液 for BDY FL Staurosporine

以下数据基于产品分子量 901.82。 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

选择批次从而根据批次分子量重新计算:
浓度/溶剂体积/质量 1 mg 5 mg 10 mg
0.1 mM 11.09 mL 55.44 mL 110.89 mL
0.5 mM 2.22 mL 11.09 mL 22.18 mL
1 mM 1.11 mL 5.54 mL 11.09 mL
5 mM 0.22 mL 1.11 mL 2.22 mL

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产品说明书 for BDY FL Staurosporine

分析证书/产品说明书
化学品安全技术说明书

参考文献 for BDY FL Staurosporine

参考文献是支持产品生物活性的出版物。

Hirozane et al (2019) Structure-based rational design of staurosporine-based fluorescent probe with broad-ranging kinase affinity for kinase panel application. Bioorg.Med.Chem.Lett. 29 126641 PMID: 31526603

If you know of a relevant reference for BDY FL Staurosporine, please let us know.

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关键词: BDY FL Staurosporine, BDY FL Staurosporine supplier, BDY, FL, Staurosporine, fluorescent, probe, BODIPY, fluorescence, probes, assay, reagents, TR-FRET, time-resolved, resonance, energy, transfer, kinases, and, FP, Assay, Reagents, Other, Fluorescent, Probes, 7985, Tocris Bioscience

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