BI dihydrochloride

Cat. No. 7466 提交评论说明书 / 分析证书（CoA） / 化学品安全技术说明书（SDS）

Pricing	Availability		Qty

说明： DFHBI derivative for imaging of RNA in living cells that bind Broccoli aptamers

化学名： (5Z)-3-(1H-Benzimidazol-7-ylmethyl)-5-[(3,5-difluoro-4-hydroxyphenyl)methylene]-3,5-dihydro-2-methyl-4H-imidazol-4-one dihydrochloride

纯度： ≥98% (HPLC)

文献 (1)

生物活性技术数据溶解性计算器说明书参考文献

生物活性

BI is a DFHBI derivative for imaging of RNA in cells. BI increases brightness and photostability of Broccoli aptamers in cells. BI enables imaging of single Broccoli-tagged mRNAs in living cells. BI exhibits peak excitation of 470 nm and peak fluorescence emission of 505 nm when bound to Broccoli. BI exhibits higher affinity for Broccoli aptamers and a 10.5-fold increase in cellular fluorescence compared with DFHBI-1T (Cat. No. 5610) (K_D values are 51 and 305 nM, respectively).

许可信息

Sold under license from Lucerna

Optical Data for BI dihydrochloride

Emission Color	Green
λ_abs	470 nm
λ_em	505 nm
Extinction Coefficient (ε)	33600 M^-1cm^-1
Quantum Yield (φ)	0.669
Cell Permeable	Yes
Application	DFHBI Derivative for Imaging of RNA in Living Cells

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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技术数据

分子量	441.26
公式	C₁₉H₁₄F₂N₄O₂.2HCl
储存	Store at -20°C
纯度	≥98% (HPLC)
CAS Number	2758438-31-0
InChI Key	YKXWKZLDMGAVJO-ZYMKCKPMSA-N
Smiles	CC1=N/C(C(N1CC2=C3NC=NC3=CC=C2)=O)=C\C4=CC(F)=C(C(F)=C4)O.Cl.Cl

上方提供的技术数据仅供参考。批次相关数据请参见分析证书。

Tocris products are intended for laboratory research use only, unless stated otherwise.

溶解性数据

	溶剂	最高浓度 mg/mL	最高浓度 mM
溶解性
	water	44.13	100
	DMSO	22.06	50

制备储备液

以下数据基于产品分子量 441.26。 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

浓度/溶剂体积/质量	1 mg	5 mg	10 mg
1 mM	2.27 mL	11.33 mL	22.66 mL
5 mM	0.45 mL	2.27 mL	4.53 mL
10 mM	0.23 mL	1.13 mL	2.27 mL
50 mM	0.05 mL	0.23 mL	0.45 mL

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产品说明书

分析证书/产品说明书

化学品安全技术说明书

参考文献

参考文献是支持产品生物活性的出版物。

Li et al (2020) Fluorophore promoted RNA folding and photostability enables imaging of single Broccoli-tagged mRNAs in live mammalian cells. Angew.Chem.Int.Ed.Engl. 59 4511 PMID: 31850609

If you know of a relevant reference for BI dihydrochloride, please let us know.

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