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说明： Terbium cryptate FRET donor for TR-FRET assay development, cysteine reactive for conjugation
生物活性 for CoraFluor™ 1, thiol reactive
CoraFluor™ 1, thiol reactive is a terbium-based time-resolved Förster resonance energy transfer (TR-FRET) donor for TR-FRET assay development, such as target engagement and ternary complex assays. This product is designed with maleimide functionality to conjugate to cysteines, making it suitable for labeling biomolecules, including antibodies, nanobodies, proteins and peptides. It is exceptionally bright, providing unmatched assay sensitivity and stability in biological media over other commercially available TR-FRET donors. CoraFluor™ 1, thiol reactive is compatible with common acceptor dyes such as FAM, TMR and Cyanine 5, SE (Cat. No. 5436), enabling seamless assay integration in high throughput screening applications.
CoraFluor™ 1, amine reactive and CoraFluor™ 2, amine reactive also available.
Please refer to the protocol for further information on conjugation.
CoraFluor™ is a trademark of Bio-Techne Corp.
许可信息
Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254
Optical Data for CoraFluor™ 1, thiol reactive
| λabs | 338 nm |
|---|---|
| Closest Laser line | 337 nm |
| Reactive Group | Maleimide |
| Reactivity | Thiols |
| Cell Permeable | No |
| Correction Factor 280 | 0.157 |
| Application | Time-resolved fluorescence energy transfer (TR-FRET) |
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectral Viewer
技术数据 for CoraFluor™ 1, thiol reactive
| 分子量 | 1441.33 |
| 储存 | Store at -20°C |
上方提供的技术数据仅供参考。批次相关数据请参见分析证书。
Tocris products are intended for laboratory research use only, unless stated otherwise.
产品说明书 for CoraFluor™ 1, thiol reactive
参考文献 for CoraFluor™ 1, thiol reactive
参考文献是支持产品生物活性的出版物。
Payne et al (2021) Bright and stable luminescent probes for target engagement profiling in live cells. Nat.Chem.Biol. 17 1168 PMID: 34675420
Payne et al (2022) A direct high-throughput protein quantification strategy facilitates discovery and characterization of a celastrol-derived BRD4 degrader. Cell Chem.Biol. 29 1333 PMID: 35649410
Payne et al (2022) Resolving the deceptive isoform and complex selectivity of HDAC1/2 inhibitors. Cell Chem.Biol. 29 1140 PMID: 35298895
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