CoraFluor™ 2, amine reactive

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说明: Terbium cryptate FRET donor for TR-FRET assay development; amine reactive for conjugation
说明书
引用文献
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文献 (2)

生物活性 for CoraFluor™ 2, amine reactive

CoraFluor™ 2, amine reactive is a terbium-based time-resolved Förster resonance energy transfer (TR-FRET) donor for TR-FRET assay development, such as target engagement and ternary complex assays. CoraFluor™ 2 is designed with a PFP ester for reactivity with primary amines, making it suitable for labeling biomolecules, including antibodies, nanobodies, proteins and peptides. CoraFluor™ 2 is cell permeable and can be used in live cell intracellular TR-FRET assays. It is exceptionally bright, providing unmatched assay sensitivity and stability in biological media over other commercially available TR-FRET donors. CoraFluor™ 2 displays red shifted excitation and is excitable by both 365 nm and 405 nm lasers, and is compatible with common acceptor dyes such as FAM/FITC, AF488, GFP and mCherry, enabling seamless assay integration in high throughput screening applications.

CoraFluor™ 1, amine reactive and CoraFluor™ 1, thiol reactive also available.

Please refer to the protocol for further information on conjugation.

CoraFluor™ is a trademark of Bio-Techne Corp.

Scientific Data

CoraFluor™ 2 stability profile. Stability profiling of CoraFluor™ 2 and a commercially available TR-FRET donor (5 nM) after 7 days of exposure to 50 mM PBS, acetic acid (pH 3), sodium acetate (pH 5), MES (pH 6) or Tris buffer (pH 7.5) supplemented with various additives (DTPA, Ca2+, Mg2+, DTT, TCEP = 1 mM; Mn2+, Cu2+ = 0.5 mM).

Binding Activity Live cell TR-FRET assay. View Larger

Live cell TR-FRET assay. Cellular dose-dependent inhibition of HDAC1 via CoraFluor™ 2 labeled HaloTag® in HEK293T cells by various HDAC inhibitors (treatment for 4 h at 37 °C, 1 μM SAHA-NCT). HaloTag is a trademark of Promega Corporation.

许可信息

Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Optical Data for CoraFluor™ 2, amine reactive

CoraFluor™ 2, amine reactive Dye Spectra
λabs 352 nm
Reactivity Primary amines
Application Time-resolved fluorescence energy transfer (TR-FRET)

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectral Viewer

Spectral Viewer

技术数据 for CoraFluor™ 2, amine reactive

分子量 1623.02
储存 Store at -20°C

上方提供的技术数据仅供参考。批次相关数据请参见分析证书。

Tocris products are intended for laboratory research use only, unless stated otherwise.

产品说明书 for CoraFluor™ 2, amine reactive

参考文献 for CoraFluor™ 2, amine reactive

参考文献是支持产品生物活性的出版物。

Payne et al (2021) Bright and stable luminescent probes for target engagement profiling in live cells. Nat.Chem.Biol. 17 1168 PMID: 34675420


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CoraFluor™ 2, amine reactive 实验方案

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