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Submit ReviewDAF FM diacetate
说明： Cell permeable photostable nitric oxide (NO) fluorescent indicator
化学名： 4-Amino-5-methylamino-2',7'-difluorofluorescein diacetate
纯度： ≥80% (HPLC)
生物活性 for DAF FM diacetate
DAF-FM DA is a cell-permeable fluorescent probe for the detection of intracellular nitric oxide (NO). Once inside the cell, it is hydrolyzed by cytosolic esterases to cell-impermeant DAF-FM. DAF-FM reacts with NO+ equivalents, such as nitric anhydride (N2O3), which are formed by autoxidation of NO. Under aerobic conditions, DAF-FM can rapidly and irreversibly trap NO to yield highly fluorescent triazolofluoresceins (DAF-Ts). DAF-FM DA is photostable and stable in pH 5.5 to 12. It can be used to quantitatively monitor agonist-evoked NO synthesis in cultured HUVECs. DAF-FM DA may also be used to quantify NO levels in induced pluripotent stem cell-derived derived cardiomyocytes (iPSC-CM).
It is recommended to prepare stock solutions in DMSO.
Optical Data for DAF FM diacetate
| Emission Color | Green |
|---|---|
| λabs | 495 nm |
| λem | 515 nm |
| Extinction Coefficient (ε) | 84000 M-1cm-1 |
| Cell Permeable | Yes |
| pH Sensitivity | Independent of pH when pH >5.8 |
| Application | Confocal Microscopy, Flow cytometry |
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技术数据 for DAF FM diacetate
| 分子量 | 496.42 |
| 公式 | C25H18F2N2O7 |
| 储存 | Store at -20°C |
| 纯度 | ≥80% (HPLC) |
| CAS Number | 254109-22-3 |
| PubChem ID | 2762646 |
| InChI Key | BEVHTVRRVVEMEF-UHFFFAOYSA-N |
| Smiles | O=C1OC2(C3=CC=C(C(N)=C13)NC)C4=CC(F)=C(C=C4OC5=CC(OC(C)=O)=C(C=C52)F)OC(C)=O |
上方提供的技术数据仅供参考。批次相关数据请参见分析证书。
Tocris products are intended for laboratory research use only, unless stated otherwise.
产品说明书 for DAF FM diacetate
参考文献 for DAF FM diacetate
参考文献是支持产品生物活性的出版物。
Kojima et al (1999) Fluorescent Indicators for Imaging Nitric Oxide Production. Angew.Chem.Int.Ed.Engl. 38 3209 PMID: 10556905
Sheng et al (2005) DAF-FM (4-amino-5-methylamino-2',7'-difluorofluorescein) diacetate detects impairment of agonist-stimulated nitric oxide synthesis by elevated glucose in human vascular endothelial cells: reversal by vitamin C and L-sepiapterin. J.Pharmacol.Exp.Ther. 315 931 PMID: 16093274
Almeida et al (2021) Sensing nitric oxide in cells: historical technologies and future outlook. ACS.Sens. 6 1695 PMID: 33871990
Xu et al (2022) Uncompensated mitochondrial oxidative stress underlies heart failure in an iPSC-derived model of congenital heart disease. Cell Stem Cell 29 840 PMID: 35395180
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Fluorescent Dyes and Probes Research Product Guide
This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:
- Fluorescent Dyes, including dyes for flow cytometry
- Fluorescent Probes and Stains, including our new MitoBrilliantTM mitochondria stains
- Tissue Clearing Kits and Reagents
- Aptamer-based RNA Imaging Reagents
- Fluorescent Probes for Imaging Bacteria
- TSA VividTM Fluorophore Kits
- TSA Reagents for Enhancing IHC, ICC & FISH Signals