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Submit ReviewDFHBI 1T
说明： GFP fluorophore mimic for imaging RNA in living cells; activated by binding Spinach2 and Broccoli aptamers
化学名： (5Z)-5-[(3,5-Difluoro-4-hydroxyphenyl)methylene]-3,5-dihydro-2-methyl-3-(2,2,2-trifluoroethyl)-4H-imidazol-4-one
纯度： ≥98% (HPLC)
生物活性 for DFHBI 1T
DFHBI 1T is a mimic of green fluorescent protein (GFP) fluorophore for imaging RNA in living cells. DFHBI 1T fluorescence is activated by binding to Spinach2 or Broccoli aptamers, also binds to Squash aptamer (Kd = 45 nM). DFHBI 1T exhibits peak excitation maxima of 482 nm and peak fluorescence emission of 505 nm when bound to Spinach2, enabling imaging with GFP filter cubes. Exhibits higher specific fluorescence and lower background fluorescence when bound to Spinach2 compared with Spinach2-DFHBI.
技术数据 for DFHBI 1T
| 分子量 | 320.21 |
| 公式 | C13H9F5N2O2 |
| 储存 | Store at -20°C |
| 纯度 | ≥98% (HPLC) |
| CAS Number | 1539318-36-9 |
| PubChem ID | 101889712 |
| InChI Key | AWYCLBWNRONMQC-WMZJFQQLSA-N |
| Smiles | O=C1N(CC(F)(F)F)C(C)=N\C1=C/C2=CC(F)=C(O)C(F)=C2 |
上方提供的技术数据仅供参考。批次相关数据请参见分析证书。
Tocris products are intended for laboratory research use only, unless stated otherwise.
溶解性数据 for DFHBI 1T
| 溶剂 | 最高浓度 mg/mL | 最高浓度 mM | |
|---|---|---|---|
| 溶解性 | |||
| DMSO | 32.02 | 100 | |
| ethanol | 16.01 | 50 |
制备储备液 for DFHBI 1T
以下数据基于产品分子量 320.21。 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| 浓度/溶剂体积/质量 | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 3.12 mL | 15.61 mL | 31.23 mL |
| 5 mM | 0.62 mL | 3.12 mL | 6.25 mL |
| 10 mM | 0.31 mL | 1.56 mL | 3.12 mL |
| 50 mM | 0.06 mL | 0.31 mL | 0.62 mL |
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产品说明书 for DFHBI 1T
参考文献 for DFHBI 1T
参考文献是支持产品生物活性的出版物。
Song et al (2014) Plug-and-play fluorophores extend the spectral properties of Spinach. J.Am.Chem.Soc. 136 198 PMID: 24393009
Filonov et al (2015) In-gel imaging of RNA processing using broccoli reveals optimal aptamer expression strategies. Chem.Biol. 22 649 PMID: 26000751
Svensen and Jaffrey (2016) Fluorescent RNA Aptamers as a Tool to Study RNA-Modifying Enzymes. Cell Chem.Biol. 23 415 PMID: 26877022
Truong et al (2022) The fluorescent aptamer Squash extensively repurposes the adenine riboswitch fold. Nat.Chem.Biol. 18 191 PMID: 34937911
Dey et al (2022) Repurposing an adenine riboswitch into a fluorogenic imaging and sensing tag. Nat.Chem.Biol. 18 180 PMID: 34937909
If you know of a relevant reference for DFHBI 1T, please let us know.
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关键词: DFHBI 1T, DFHBI 1T supplier, DFHBI1T, fluorescence, fluorophores, RNA, imaging, Spinach2, localization, green, fluorescent, protein, Broccoli, light, up, aptamers, FLAPs, Aptamer-based, Imaging, Fluorescent, Probes, 5610, Tocris Bioscience
4 篇 DFHBI 1T 的引用文献
引用文献是使用了 Tocris 产品的出版物。 DFHBI 1T 的部分引用包括：
Paul S et al (2022) Specific Nucleic AcId Ligation for the detection of Schistosomes: SNAILS. PLoS Negl Trop Dis 16 e0010632 PMID: 35881651
Robert et al (2022) Quantitative RNA imaging in single live cells reveals age-dependent asymmetric inheritance. Cell Rep 41 111656 PMID: 36384120
Jeong Wook et al (2020) Cell-free biosensors for rapid detection of water contaminants. Nat Biotechnol 38 1451-1459 PMID: 32632301
Yim et al (2019) Multiplex transcriptional characterizations across diverse bacterial species using cell-free systems. Mol Syst Biol 15 e8875 PMID: 31464371
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该领域的文献
Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!
*请注意，Tocris 仅会向正规科研企业/机构地址发送文献。
Fluorescent Dyes and Probes Research Product Guide
This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:
- Fluorescent Dyes, including dyes for flow cytometry
- Fluorescent Probes and Stains, including our new MitoBrilliantTM mitochondria stains
- Tissue Clearing Kits and Reagents
- Aptamer-based RNA Imaging Reagents
- Fluorescent Probes for Imaging Bacteria
- TSA VividTM Fluorophore Kits
- TSA Reagents for Enhancing IHC, ICC & FISH Signals