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DFHBI

Cat. No. 5609 提交评论 说明书 / 分析证书（CoA） / 化学品安全技术说明书（SDS）
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说明： GFP fluorophore mimic for imaging RNA in living cells; activated by binding Spinach2 and Broccoli aptamers
化学名： (5Z)-5-[(3,5-Difluoro-4-hydroxyphenyl)methylene]-3,5-dihydro-2,3-dimethyl-4H-imidazol-4-one
纯度： ≥98% (HPLC)
说明书
引用文献
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文献 (1)
生物活性 技术数据 溶解性 计算器 说明书 参考文献

生物活性 for DFHBI

DFHBI is a mimic of green fluorescent protein (GFP) fluorophore for imaging RNA in living cells. DFHBI fluorescence is activated by binding to Spinach2 or Broccoli aptamers. Exhibits peak excitation maxima of 447 nm and peak fluorescence emission of 501 nm when bound to Spinach2.

技术数据 for DFHBI

分子量 252.22
公式 C12H10F2N2O2
储存 Store at -20°C
纯度 ≥98% (HPLC)
CAS Number 1241390-29-3
PubChem ID 70808995
InChI Key ZDDIJYXDUBFLID-YHYXMXQVSA-N
Smiles CN(C2=O)C(C)=N\C2=C/C1=CC(F)=C(O)C(F)=C1

上方提供的技术数据仅供参考。批次相关数据请参见分析证书。

Tocris products are intended for laboratory research use only, unless stated otherwise.

溶解性数据 for DFHBI

溶剂 最高浓度 mg/mL 最高浓度 mM
溶解性
DMSO 25.22 100

制备储备液 for DFHBI

以下数据基于产品分子量 252.22。 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

选择批次从而根据批次分子量重新计算:
浓度/溶剂体积/质量 1 mg 5 mg 10 mg
1 mM 3.96 mL 19.82 mL 39.65 mL
5 mM 0.79 mL 3.96 mL 7.93 mL
10 mM 0.4 mL 1.98 mL 3.96 mL
50 mM 0.08 mL 0.4 mL 0.79 mL

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参考文献 for DFHBI

参考文献是支持产品生物活性的出版物。

Song et al (2014) Plug-and-play fluorophores extend the spectral properties of Spinach. J.Am.Chem.Soc. 136 198 PMID: 24393009

Filonov et al (2015) In-gel imaging of RNA processing using broccoli reveals optimal aptamer expression strategies. Chem.Biol. 22 649 PMID: 26000751

Strack et al (2014) Using Spinach-based sensors for fluorescence imaging of intracellular metabolites and proteins in living bacteria. Nat.Protoc. 9 146 PMID: 24356773

Paige et al (2011) RNA mimics of green fluorescent protein. Science 333 642 PMID: 21798953

Svensen and Jaffrey (2016) Fluorescent RNA Aptamers as a Tool to Study RNA-Modifying Enzymes. Cell Chem.Biol. 23 415 PMID: 26877022

Truong et al (2022) The fluorescent aptamer Squash extensively repurposes the adenine riboswitch fold. Nat.Chem.Biol. 18 191 PMID: 34937911

If you know of a relevant reference for DFHBI, please let us know.

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关键词: DFHBI, DFHBI supplier, fluorescence, fluorophores, RNA, imaging, Spinach2, localization, green, fluorescent, protein, Broccoli, light, up, aptamers, FLAPs, Aptamer-based, Imaging, Fluorescent, Probes, 5609, Tocris Bioscience

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该领域的文献

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Fluorescent Dyes and Probes Research Product Guide

Fluorescent Dyes and Probes Research Product Guide

This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:

  • Fluorescent Dyes, including dyes for flow cytometry
  • Fluorescent Probes and Stains, including our new MitoBrilliantTM mitochondria stains
  • Tissue Clearing Kits and Reagents
  • Aptamer-based RNA Imaging Reagents
  • Fluorescent Probes for Imaging Bacteria
  • TSA VividTM Fluorophore Kits
  • TSA Reagents for Enhancing IHC, ICC & FISH Signals