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说明： Voltage-sensitive probe; used to detect changes in membrane potential in electrophysiology protocols
化学名： 6-[2-(N,N-Dibutylamino)naphthyl]ethenyl-4'-pyridinium propanesulfonate
纯度： ≥90% (HPLC)
生物活性 for Di 4 ANEPPS
Di 4 ANEPPS is a voltage-sensitive dye, also known as an electrochromic probe, which fluoresces in response to change in electrical potential across a cellular membrane. Di 4 ANEPPS fluoresces only when membrane bound; change in electrical potential causes an intramolecular charge transfer and blue-shift in the excitation spectra. Di 4 ANEPPS is commonly used to detect transient changes in membrane potential in excitable cells such as neurons and cardiac cells. Excitation ratio measurements can be used to determine membrane potential.
In model phospholipid membranes Ex/Em maxima are 465/635 nm, however spectral properties are highly dependent on membrane environment.
It is recommended to prepare stock solutions in DMSO.
Optical Data for Di 4 ANEPPS
| Emission Color | Red |
|---|---|
| λabs | 465 nm |
| λem | 635 nm |
| Application | Electrophysiology, Fluorescence Microscopy |
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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技术数据 for Di 4 ANEPPS
| 分子量 | 480.67 |
| 公式 | C28H36N2O3S |
| 储存 | Store at -20°C |
| 纯度 | ≥90% (HPLC) |
| CAS Number | 90134-00-2 |
| PubChem ID | 6438327 |
| InChI Key | HAPJROQJVSPKCJ-UHFFFAOYSA-N |
| Smiles | [O-]S(CCC[N+](C=C1)=CC=C1/C=C/C(C=C2C=C3)=CC=C2C=C3N(CCCC)CCCC)(=O)=O |
上方提供的技术数据仅供参考。批次相关数据请参见分析证书。
Tocris products are intended for laboratory research use only, unless stated otherwise.
产品说明书 for Di 4 ANEPPS
参考文献 for Di 4 ANEPPS
参考文献是支持产品生物活性的出版物。
Loew et al (1992) A naphthyl analog of the aminostyryl pyridinium class of potentiometric membrane dyes shows consistent sensitivity in a variety of tissue, cell, and model membrane preparations. J.Membr.Biol. 130 1 PMID: 1469705
Tominga & Tominga (2013) A new nonscanning confocal microscopy module for functional voltage-sensitive dye and Ca2+ imaging of neuronal circuit activity. J.Neurophysiol. 110 553 PMID: 23615547
Asamoah et al (2003) A fluorometric approach to local electric field measurements in a voltage-gated ion channel. Neuron. 37 85 PMID: 12526775
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