DMHBO+

Cat. No. 7764 提交评论说明书 / 分析证书（CoA） / 化学品安全技术说明书（SDS）

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说明： Fluorescent upon binding to Chili aptamer; for imaging RNA in cells

化学名： 4-(4-((Z)-4-Hydroxy-3,5-dimethoxybenzylidene)-2-((hydroxyimino)methyl)-5-oxo-4,5-dihydro-1H-imidazol-1-yl)-N,N,N-trimethylbenzenaminium iodide

纯度： ≥98% (HPLC)

说明书

引用文献

文献 (1)

生物活性技术数据溶解性计算器说明书参考文献

生物活性 for DMHBO+

DMHBO+ is a cationic fluorophore. Its fluorescence is activated by binding to chili aptamer (Kd = 12 nM). Chili-DMHBO+ complex mimics red fluorescent proteins, suitable for imaging RNA in cells. It is an ideal FRET donor to the rhodamine dye Atto 590 and has applications in FRET-based analytical RNA systems. Excitation/emission maxima (λ) = 456/592 nm; quantum yield = 0.1; stokes shift = 136 nm.

Optical Data for DMHBO+

λ_abs	456 nm
λ_em	592 nm
Quantum Yield (φ)	0.1
Application	Time-resolved fluorescence energy transfer (TR-FRET)

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Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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技术数据 for DMHBO+

分子量	552.37
公式	C₂₂H₂₅IN₄O₅
储存	Store at -20°C
纯度	≥98% (HPLC)
CAS Number	2322286-81-5
InChI Key	KRIJKRIPLDZXHI-UHFFFAOYSA-N
Smiles	O/N=C/C(N(C1=CC=C(C=C1)[N+](C)(C)C)C/2=O)=NC2=C/C3=CC(OC)=C(C(OC)=C3)O.[I-]

上方提供的技术数据仅供参考。批次相关数据请参见分析证书。

Tocris products are intended for laboratory research use only, unless stated otherwise.

溶解性数据 for DMHBO+

	溶剂	最高浓度 mg/mL	最高浓度 mM
溶解性
溶解性	DMSO	27.62	50

制备储备液 for DMHBO+

以下数据基于产品分子量 552.37。 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

浓度/溶剂体积/质量	1 mg	5 mg	10 mg
0.5 mM	3.62 mL	18.1 mL	36.21 mL
2.5 mM	0.72 mL	3.62 mL	7.24 mL
5 mM	0.36 mL	1.81 mL	3.62 mL
25 mM	0.07 mL	0.36 mL	0.72 mL

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产品说明书 for DMHBO+

分析证书/产品说明书

化学品安全技术说明书

参考文献 for DMHBO+

参考文献是支持产品生物活性的出版物。

Steinmetzger et al (2019) A multicolor large stokes shift fluorogen-activating RNA aptamer with cationic chromophores. Chemistry 25 1931 PMID: 30485561

If you know of a relevant reference for DMHBO+, please let us know.

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关键词: DMHBO+, DMHBO+ supplier, fluorescent, RNA, imaging, red, fluorophore, light-up, aptamers, FLAPs, aptamer-based, probes, Aptamer-based, Imaging, Fluorescent, Probes, 7764, Tocris Bioscience

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Fluorescent Dyes and Probes Research Product Guide

This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:

Fluorescent Dyes, including dyes for flow cytometry
Fluorescent Probes and Stains, including our new MitoBrilliant^TM mitochondria stains
Tissue Clearing Kits and Reagents
Aptamer-based RNA Imaging Reagents
Fluorescent Probes for Imaging Bacteria
TSA Vivid^TM Fluorophore Kits
TSA Reagents for Enhancing IHC, ICC & FISH Signals