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E 64

Cat. No. 5208 4 篇引用文献 1 条评论 提交评论 说明书 / 分析证书（CoA） / 化学品安全技术说明书（SDS）
Pricing Availability   Qty
说明： Potent and irreversible cysteine protease inhibitor
化学名： (2S,3S)-3-[[[(1S)-1-[[[4-(Aminoiminomethyl)amino]butyl]amino]carbonyl]-3-methylbutyl]amino]carbonyl]-2-oxiranecarboxylic acid
纯度： ≥98% (HPLC)
说明书
引用文献 (4)
评论 (1)
生物活性 技术数据 溶解性 计算器 说明书 参考文献

生物活性 for E 64

E 64 is a potent and irreversible cysteine protease inhibitor (IC50 values are 1.4, 2.5 and 4.1 nM for cathepsin K, L and S respectively. Also inhibits papain, calpain, cathepsin B and cathepsin H. Has no effect on serine proteases. Reduces HSC-3 tongue cancer cell invasion and induces oxidative stress and apoptosis in filarial parasites. Also inhibits autophagy-associated lysosomal degradation in vitro.

技术数据 for E 64

分子量 357.41
公式 C15H27N5O5
储存 Store at -20°C
纯度 ≥98% (HPLC)
CAS Number 66701-25-5
PubChem ID 123985
InChI Key LTLYEAJONXGNFG-DCAQKATOSA-N
Smiles O=[C@@](N[C@H]([C@](NCCCCNC(N)=N)=O)CC(C)C)[C@@H]1[C@@H](C(O)=O)O1

上方提供的技术数据仅供参考。批次相关数据请参见分析证书。

Tocris products are intended for laboratory research use only, unless stated otherwise.

溶解性数据 for E 64

溶剂 最高浓度 mg/mL 最高浓度 mM
溶解性
water 7.15 20

制备储备液 for E 64

以下数据基于产品分子量 357.41。 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

选择批次从而根据批次分子量重新计算:
浓度/溶剂体积/质量 1 mg 5 mg 10 mg
0.2 mM 13.99 mL 69.95 mL 139.9 mL
1 mM 2.8 mL 13.99 mL 27.98 mL
2 mM 1.4 mL 6.99 mL 13.99 mL
10 mM 0.28 mL 1.4 mL 2.8 mL

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产品说明书 for E 64

分析证书/产品说明书
选择另一批次：
化学品安全技术说明书

参考文献 for E 64

参考文献是支持产品生物活性的出版物。

Susa et al (2004) Human primary osteoclasts: in vitro generation and applications as pharmacological and clinical assay. J.Transl.Med. 2 6 PMID: 15025786

Bitu et al (2013) Cathepsin K is present in invasive oral tongue squamous cell carcinoma in vivo and in vitro. PLoS ONE 8 e70925 PMID: 23951042

Wadhawan et al (2014) Inhibition of cathepsin B by E-64 induces oxidative stress and apoptosis in filarial parasite. PLoS ONE 9 e93161 PMID: 24667798

Zhao et al (2010) Cytosolic FoxO1 is essential for the induction of autophagy and tumour suppressor activity. Nat.Cell.Biol. 12 665 PMID: 20543840

If you know of a relevant reference for E 64, please let us know.

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关键词: E 64, E 64 supplier, E64, potent, irreversible, natural, cysteine, protease, inhibitors, inhibits, cathepsin, K, L, S, B, H, calpain, papain, Cathepsin, Calpains, Calcium-Sensitive, Proteases, 5208, Tocris Bioscience

4 篇 E 64 的引用文献

引用文献是使用了 Tocris 产品的出版物。 E 64 的部分引用包括：

Lee et al (2015) Tricyclic Antidepressants amitript. and desipr. Induced Neurotoxicity Associated with Parkinson's Disease. PLoS One 38 734 PMID: 26242194

Min et al (2021) A recombinant bovine adenoviral mucosal vaccine expressing mycobacterial antigen-85B generates robust protection against tuberculosis in mice. Cell Rep Med 2 100372 PMID: 34467249

Xianglan et al (2019) Apolipoprotein E is a concentration-dependent pulmonary danger signal that activates the NLRP3 inflammasome and IL-1β secretion by bronchoalveolar fluid macrophages from asthmatic subjects. J Allergy Clin Immunol 144 426-441.e3 PMID: 30872118

Bénédicte et al (2023) Microglial P2X4 receptors promote ApoE degradation and contribute to memory deficits in Alzheimer's disease. Cell Mol Life Sci 80 138 PMID: 37145189

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E 64 的评论

平均评分: 4 (Based on 1 Review.)

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Reduction of cathepsin acitivity in vitro with E64 inhibitor.
By Yann Malato on 10/03/2024
分析类型： In Vitro
种属： Human
细胞系/组织： ARPE19

I used the E64 in ARPE19 cells after sodium iodate administration with a fluorescent cathepsin sensor (magic red). I used E64 at 10uM and it reduced the signal of magic red vs DMSO 0.01% control