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Submit ReviewFFN 200 dihydrochloride is a selective fluorescent VMAT2 substrate. Selectively traces exocytosis in dopaminergic neuronal cell culture and brain tissue. Excitation and emission maxima are 352 and 451 nm, respectively.
Sold with the permission of Columbia University.
FFN200 in vesicle clusters in axons of cultured dopaminergic neurons, and exocytosis upon high potassium stimulation (time lapse acquisition at 12 images/min).
分子量 | 277.15 |
公式 | C11H12N2O2.2HCl |
储存 | Store at -20°C |
纯度 | ≥98% (HPLC) |
CAS Number | 2080306-27-8 |
PubChem ID | 124201850 |
InChI Key | YNQXRSNAOWBGLD-UHFFFAOYSA-N |
Smiles | NC1=CC(OC(C=C2CCN)=O)=C2C=C1.Cl.Cl |
上方提供的技术数据仅供参考。批次相关数据请参见分析证书。
Tocris products are intended for laboratory research use only, unless stated otherwise.
溶剂 | 最高浓度 mg/mL | 最高浓度 mM | |
---|---|---|---|
溶解性 | |||
water | 27.71 | 100 | |
DMSO | 27.71 | 100 |
以下数据基于产品分子量 277.15。 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
浓度/溶剂体积/质量 | 1 mg | 5 mg | 10 mg |
---|---|---|---|
1 mM | 3.61 mL | 18.04 mL | 36.08 mL |
5 mM | 0.72 mL | 3.61 mL | 7.22 mL |
10 mM | 0.36 mL | 1.8 mL | 3.61 mL |
50 mM | 0.07 mL | 0.36 mL | 0.72 mL |
参考文献是支持产品生物活性的出版物。
Pereira et al (2015) Fluorescent false neurotransmitter reveals functionally silent DA vesicle clusters in the striatum. Nat.Neurosci. 19 578 PMID: 26900925
Li (2017) Probes for monitoring regulated exocytosis. Cell Calcium 64 65 PMID: 28089267
If you know of a relevant reference for FFN 200 dihydrochloride, please let us know.
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引用文献是使用了 Tocris 产品的出版物。 FFN 200 dihydrochloride 的部分引用包括:
Matthias et al (2022) Endogenously produced catecholamines improve the regulatory function of TLR9-activated B cells. PLoS Biol 20 e3001513 PMID: 35073310
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