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RNA Imaging Probe 1c

Cat. No. 8813 Submit a Review Datasheet / COA / SDS
Pricing Availability   Qty
Description: Fluorogenic RNA imaging probe
Chemical Name: 3-[(E)-2-(1-Methylpyridin-1-ium-4-yl)ethenyl]indolizine iodide
Purity: ≥98% (HPLC)
Datasheet
Citations
Reviews
Literature (1)
Biological Activity Scientific Data Technical Data Solubility Calculators Datasheets References

Biological Activity for RNA Imaging Probe 1c

RNA Imaging Probe 1c is a fluorogenic RNA imaging probe. RNA Imaging Probe 1c displays high membrane permeability, strong fluorogenic responses upon binding RNA, compatibility with fluorescence lifetime imaging microscopy (FLIM), low cytotoxicity, and excellent photostability. Excitation and emission maxima (λ) are 556 nm and 608 nm, respectively; quantum yield = 0.49; extinction coefficient = 27,500 M-1cm-1. Suitable for live cell imaging.

Scientific Data

Confocal Microscopy Live HeLa cells labeled with RNA Imaging Probe 1c View Larger

Application of RNA Imaging Probe 1c on live HeLa cells. Confocal fluorescence images of live HeLa cells imaged with 20 μM methyl pyridinium indole (MPI) (green) ( lambda ex = 470 nm, lambda em = 525−555 nm) and 20 μM RNA Imaging Probe 1c (red) ( lambda ex = 550 nm, lambda em = 580−620 nm) for 30 min. Scale bar: 10 μm.

Confocal Microscopy DAPI counterstaining alongside RNA Imaging Probe 1c on HeLa cells View Larger

Application of RNA Imaging Probe 1c on PFA-fixed HeLa cells. Confocal fluorescence images of PFA-fixed HeLa cells incubated with 20 μM RNA Imaging Probe 1c (red) and 1 μg/mL Hoechst 33342 (blue) for 30 min.

Confocal Microscopy HeLa cells stained with RNA Imaging Probe 1c View Larger

Application of RNA Imaging Probe 1c on HeLa cells. Zoomed-in confocal fluorescence image of HeLa cells stained with 20 μM RNA Imaging Probe 1c for 30 min. Image shows the sub-nucleolar components fibrillar center (FC) (indicated by the white arrowhead), dense fibrillar component (DFC), and outer granular component (GC) (indicated by the white arrows). Scale bar: 3 μm.

Fluorescence Imaging Yeast RNA and spermine labeled with RNA Imaging Probe 1c View Larger

Yeast RNA and spermine labeled with RNA Imaging Probe 1c. Torula yeast RNA and spermine were incubated with RNA Imaging Probe 1c (10 μM). FLIM images show the formation of spherical coacervate droplets upon mixing the RNA and spermine. Image is enlarged by 1× (on the left) or by 8× (on the right). Scale bar: 10 μm.

Licensing Information

Sold under license from the University of Southern California.

Optical Data for RNA Imaging Probe 1c

λabs 556 nm
λem 608 nm
Extinction Coefficient (ε) 27500 M-1cm-1
Quantum Yield (φ) 0.49
Cell Permeable Yes

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectral Viewer

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Technical Data for RNA Imaging Probe 1c

M. Wt 362.21
Formula C16H15IN2
Storage Store at -20°C
Purity ≥98% (HPLC)
PubChem ID 171378441
InChI Key ODMXTRDMWRHIDS-UHFFFAOYSA-M
Smiles C[N+]1=CC=C(/C=C/C2=CC=C3N2C=CC=C3)C=C1.[I-]

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for RNA Imaging Probe 1c

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 7.24 20

Preparing Stock Solutions for RNA Imaging Probe 1c

The following data is based on the product molecular weight 362.21. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.2 mM 13.8 mL 69.02 mL 138.04 mL
1 mM 2.76 mL 13.8 mL 27.61 mL
2 mM 1.38 mL 6.9 mL 13.8 mL
10 mM 0.28 mL 1.38 mL 2.76 mL

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Product Datasheets for RNA Imaging Probe 1c

Certificate of Analysis / Product Datasheet
Safety Datasheet

References for RNA Imaging Probe 1c

References are publications that support the biological activity of the product.

Kim et al (2023) Development of highly fluorogenic styrene probes for visualizing RNA in live cells. ACS Chem.Biol. 18 1523 PMID: 37200527

If you know of a relevant reference for RNA Imaging Probe 1c, please let us know.

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Citations for RNA Imaging Probe 1c

Citations are publications that use Tocris products.

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Literature in this Area

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Fluorescent Dyes and Probes Research Product Guide

Fluorescent Dyes and Probes Research Product Guide

This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:

  • Fluorescent Dyes, including dyes for flow cytometry
  • Fluorescent Probes and Stains, including our new MitoBrilliantTM mitochondria stains
  • Tissue Clearing Kits and Reagents
  • Aptamer-based RNA Imaging Reagents
  • Fluorescent Probes for Imaging Bacteria
  • TSA VividTM Fluorophore Kits
  • TSA Reagents for Enhancing IHC, ICC & FISH Signals