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Submit ReviewFluorescein Tyramide is a green fluorescent reagent widely used for tyramide signal amplification (TSA) in IHC, ICC, FISH and multicolor FISH. HRP catalyzes localized deposition of multiple tyramide molecules (catalyzed reporter deposition, CARD), binding the fluorescein tyramide to adjacent tyrosines to enhance fluorescent signal.
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectral Viewer分子量 | 495.48 |
公式 | C29H21NO7 |
储存 | Store at -20°C |
纯度 | ≥95% (HPLC) |
CAS Number | 210236-90-1 |
InChI Key | MJQMGAFVBOOBIB-UHFFFAOYSA-N |
Smiles | OC1=CC2=C(C3(C4=CC=C(C=C4O2)O)C5=CC=C(C(NCCC6=CC=C(C=C6)O)=O)C=C5C(O3)=O)C=C1.OC7=CC8=C(C9(C%10=CC=C(C=C%10O8)O)C%11=CC(C(NCCC%12=CC=C(C=C%12)O)=O)=CC=C%11C(O9)=O)C=C7 |
上方提供的技术数据仅供参考。批次相关数据请参见分析证书。
Tocris products are intended for laboratory research use only, unless stated otherwise.
溶剂 | 最高浓度 mg/mL | 最高浓度 mM | |
---|---|---|---|
溶解性 | |||
DMSO | 11.89 | 24 |
以下数据基于产品分子量 495.48。 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
浓度/溶剂体积/质量 | 1 mg | 5 mg | 10 mg |
---|---|---|---|
0.24 mM | 8.41 mL | 42.05 mL | 84.09 mL |
1.2 mM | 1.68 mL | 8.41 mL | 16.82 mL |
2.4 mM | 0.84 mL | 4.2 mL | 8.41 mL |
12 mM | 0.17 mL | 0.84 mL | 1.68 mL |
参考文献是支持产品生物活性的出版物。
Karkmann et al (1999) Enzymatic signal amplification for sensitive detection of intracellular antigens by flow cytometry. J.Immunol.Methods 230 113 PMID: 10594358
Schriml et al (1999) Tyramide signal amplification (TSA)-FISH applied to mapping PCR-labeled probes less than 1 kb in size. Biotechniques 27 608 PMID: 10489619
Hopman et al (1998) Rapid synthesis of biotin-, digoxigenin-, trinitrophenyl-, and fluorochrome-labeled tyramides and their application for in situ hybridization using CARD amplification. J.Histochem.Cytochem. 46 771 PMID: 9603790
van Gijlswijk et al (1997) Fluorochrome-labeled tyramides: use in immunocytochemistry and fluorescence in situ hybridization. J.Histochem.Cytochem. 45 375 PMID: 9071319
Bobrow et al (1989) Catalyzed reporter deposition, a novel method of signal amplification. Application to immunoassays. J.Immunol.Methods 125 279 PMID: 2558138
If you know of a relevant reference for Fluorescein Tyramide, please let us know.
关键词: Fluorescein Tyramide, Fluorescein Tyramide supplier, tyramide, signal, amplification, TSA, horseradish, peroxidase, HRP, green, Tyramide, Signaling, Amplification, (TSA), 6456, Tocris Bioscience
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